biostudies-literatureCalanca NConselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - BrasilFundação de Amparo à Pesquisa do Estado de São PauloNCI NIH HHS741-750https://www.ebi.ac.uk/biostudies/studies/S-EPMC6615536biostudies-literatureUnknown14(8)Alternative protein-coding transcripts of the <i>RASSF1</i> gene have been associated with dual functions in human cancer: while <i>RASSF1C</i> isoform has oncogenic properties, <i>RASSF1A</i> is a tumour suppressor frequently silenced by hypermethylation. Recently, the antisense long non-coding RNA RASSF1 (<i>ANRASSF1)</i> was implicated in a <i>locus</i>-specific mechanism for the <i>RASSF1A</i> epigenetic repression mediated by PRC2 (Polycomb Repressive Complex 2). Here, we evaluated the methylation patterns of the promoter regions of <i>RASSF1A</i> and <i>RASSF1C</i> and the expression levels of these <i>RASSF1</i> transcripts in breast cancer and breast cancer cell lines. As expected, <i>RASSF1C</i> remained unmethylated and <i>RASSF1A</i> was hypermethylated at high frequencies in 75EpigeneticsThe long non-coding RNA <i>ANRASSF1</i> in the regulation of alternative protein-coding transcripts <i>RASSF1A</i> and <i>RASSF1C</i> in human breast cancer cells: implications to epigenetic therapy.PMC6615536Finance Code 001CNPq ProfixFAPESP grant# 07/59110-9P30 CA051008Oliveira RAPaschoal APGalindo LTMunhoz EPCaldeira JRFRogatto SRCalanca NBarbosa BMCavalli LRRainho CAfalseThe long non-coding RNA <i>ANRASSF1</i> in the regulation of alternative protein-coding transcripts <i>RASSF1A</i> and <i>RASSF1C</i> in human breast cancer cells: implications to epigenetic therapy.Alternative protein-coding transcripts of the <i>RASSF1</i> gene have been associated with dual functions in human cancer: while <i>RASSF1C</i> isoform has oncogenic properties, <i>RASSF1A</i> is a tumour suppressor frequently silenced by hypermethylation. Recently, the antisense long non-coding RNA RASSF1 (<i>ANRASSF1)</i> was implicated in a <i>locus</i>-specific mechanism for the <i>RASSF1A</i> epigenetic repression mediated by PRC2 (Polycomb Repressive Complex 2). Here, we evaluated the methylation patterns of the promoter regions of <i>RASSF1A</i> and <i>RASSF1C</i> and the expression levels of these <i>RASSF1</i> transcripts in breast cancer and breast cancer cell lines. As expected, <i>RASSF1C</i> remained unmethylated and <i>RASSF1A</i> was hypermethylated at high frequencies in 752019-01-01T00:00:00Z2019 Aug2025-04-18T22:11:27.924Z2020-05-30T07:02:25ZS-EPMC66155363106266010.1080/15592294.2019.1615355