{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Barsakis K"],"funding":["NINDS NIH HHS","National Institutes of Health"],"pagination":["437-448"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6622178"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["80(7)"],"pubmed_abstract":["Next Generation Sequencing allows for testing and typing of entire genes of the HLA region. A better and comprehensive sequence assessment can be achieved by the inclusion of full gene sequences of all the common alleles at a given locus. The common alleles of DRB5 are under-characterized with the full exon-intron sequence of two alleles available. In the present study the DRB5 genes from 18 subjects alleles were cloned and sequenced; haplotype analysis showed that 17 of them had a single copy of DRB5 and one consanguineous subject was homozygous at all HLA loci. Methodological approaches including robust and efficient long-range PCR amplification, molecular cloning, nucleotide sequencing and de novo sequence assembly were combined to characterize DRB5 alleles. DRB5 sequences covering from"],"journal":["Human immunology"],"pubmed_title":["Complete nucleotide sequence characterization of DRB5 alleles reveals a homogeneous allele group that is distinct from other DRB genes."],"pmcid":["PMC6622178"],"funding_grant_id":["U19NS095774","U19 NS095774"],"pubmed_authors":["Pickle W","Barsakis K","Chi A","Babrzadeh F","Mallempati K","Mindrinos M","Fernandez-Vina MA"],"additional_accession":[]},"is_claimable":false,"name":"Complete nucleotide sequence characterization of DRB5 alleles reveals a homogeneous allele group that is distinct from other DRB genes.","description":"Next Generation Sequencing allows for testing and typing of entire genes of the HLA region. A better and comprehensive sequence assessment can be achieved by the inclusion of full gene sequences of all the common alleles at a given locus. The common alleles of DRB5 are under-characterized with the full exon-intron sequence of two alleles available. In the present study the DRB5 genes from 18 subjects alleles were cloned and sequenced; haplotype analysis showed that 17 of them had a single copy of DRB5 and one consanguineous subject was homozygous at all HLA loci. Methodological approaches including robust and efficient long-range PCR amplification, molecular cloning, nucleotide sequencing and de novo sequence assembly were combined to characterize DRB5 alleles. DRB5 sequences covering from","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jul","modification":"2026-04-12T23:59:03.247Z","creation":"2020-07-04T07:17:01Z"},"accession":"S-EPMC6622178","cross_references":{"pubmed":["30954494"],"doi":["10.1016/j.humimm.2019.04.001"]}}