biostudies-literatureHaramati SEuropean Research Council14191-203https://www.ebi.ac.uk/biostudies/studies/S-EPMC6623664biostudies-literatureUnknown31(40)The etiology and pathophysiology of anxiety and mood disorders is linked to inappropriate regulation of the central stress response. To determine whether microRNAs have a functional role in the regulation of the stress response, we inactivated microRNA processing by a lentiviral-induced local ablation of the Dicer gene in the central amygdala (CeA) of adult mice. CeA Dicer ablation induced a robust increase in anxiety-like behavior, whereas manipulated neurons survive and appear to exhibit normal gross morphology in the time period examined. We also observed that acute stress in wild-type mice induced a differential expression profile of microRNAs in the amygdala. Bioinformatic analysis identified putative gene targets for these stress-responsive microRNAs, some of which are known to be asThe Journal of neuroscience : the official journal of the Society for NeuroscienceMicroRNA as repressors of stress-induced anxiety: the case of amygdalar miR-34.PMC6623664260463Haramati SChen ANavon IGil SZwang REzra-Nevo GIssler OHornstein EfalseMicroRNA as repressors of stress-induced anxiety: the case of amygdalar miR-34.The etiology and pathophysiology of anxiety and mood disorders is linked to inappropriate regulation of the central stress response. To determine whether microRNAs have a functional role in the regulation of the stress response, we inactivated microRNA processing by a lentiviral-induced local ablation of the Dicer gene in the central amygdala (CeA) of adult mice. CeA Dicer ablation induced a robust increase in anxiety-like behavior, whereas manipulated neurons survive and appear to exhibit normal gross morphology in the time period examined. We also observed that acute stress in wild-type mice induced a differential expression profile of microRNAs in the amygdala. Bioinformatic analysis identified putative gene targets for these stress-responsive microRNAs, some of which are known to be as2011-01-01T00:00:00Z2011 Oct2025-04-04T15:02:23.784Z2019-07-30T07:04:32ZS-EPMC66236642197650410.1523/jneurosci.1673-11.201110.1523/JNEUROSCI.1673-11.2011