{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Caisova L"],"funding":["German Research Foundation"],"pagination":["74"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6624896"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15"],"pubmed_abstract":["<h4>Background</h4>Green plants comprise two lineages: (1) the streptophytes that colonised land and (2) the chlorophytes that have adaptations to land but remained mostly aquatic. To better understand what made streptophytes so successful, we are currently establishing the chlorophyte alga <i>Draparnaldia</i> sp. (Chaetophorales, Chlorophyceae) as a model for comparative analyses between these two lineages. However, establishing <i>Draparnaldia</i> as a valuable model requires that it can be transformed. Thus, our goal is to develop a transformation protocol for this alga.<h4>Results</h4>We have established the first transformation protocol for <i>Draparnaldia</i>. This protocol is based on protoplast transformation by electroporation. It includes instructions on protoplast isolation, reg"],"journal":["Plant methods"],"pubmed_title":["Regeneration and transient gene expression in protoplasts of <i>Draparnaldia</i> (chlorophytes), an emerging model for comparative analyses with basal streptophytes."],"pmcid":["PMC6624896"],"funding_grant_id":["DFG grant CA 1321/3-1"],"pubmed_authors":["Caisova L","Jobe TO"],"additional_accession":[]},"is_claimable":false,"name":"Regeneration and transient gene expression in protoplasts of <i>Draparnaldia</i> (chlorophytes), an emerging model for comparative analyses with basal streptophytes.","description":"<h4>Background</h4>Green plants comprise two lineages: (1) the streptophytes that colonised land and (2) the chlorophytes that have adaptations to land but remained mostly aquatic. To better understand what made streptophytes so successful, we are currently establishing the chlorophyte alga <i>Draparnaldia</i> sp. (Chaetophorales, Chlorophyceae) as a model for comparative analyses between these two lineages. However, establishing <i>Draparnaldia</i> as a valuable model requires that it can be transformed. Thus, our goal is to develop a transformation protocol for this alga.<h4>Results</h4>We have established the first transformation protocol for <i>Draparnaldia</i>. This protocol is based on protoplast transformation by electroporation. It includes instructions on protoplast isolation, reg","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-05-27T12:08:55.619Z","creation":"2019-07-30T07:05:10Z"},"accession":"S-EPMC6624896","cross_references":{"pubmed":["31338114"],"doi":["10.1186/s13007-019-0460-6"]}}