biostudies-literatureDeng ZScience and Technology Planning Project of Hunan ProvinceNatural Science Foundation of Hunan ProvinceNational Natural Science Foundation of China217-224https://www.ebi.ac.uk/biostudies/studies/S-EPMC6625970biostudies-literatureUnknown17Overexpression of endogenous H<sub>2</sub>S is one of the key characteristic in colon cancer. However, developing endogenous H<sub>2</sub>S-activated optical probes for specific diagnosis of colorectal cancer is rarely explored. Herein, an in situ H<sub>2</sub>S-activatable second near-infrared (NIR-II)-emitting nanoprobe based on Ag-chicken egg white (Ag-CEW) complex for intelligently lighting up colorectal cancer was explored. The designed Ag-CEW complex holds efficient NIR-II emission of 1,000-1,400 nm via endogenous H<sub>2</sub>S-induced in situ chemical reaction to form Ag<sub>2</sub>S quantum dots (QDs). After reaction, the designed Ag-CEW complex with high photo-stability and biocompatibility was successfully used for NIR-II imaging-guided specific visualization and precise locatioiScienceEndogenous H<sub>2</sub>S-Triggered In Situ Synthesis of NIR-II-Emitting Nanoprobe for In Vivo Intelligently Lighting Up Colorectal Cancer.PMC6625970216710642019JJ100022017RS3031Liu HZeng SLi YHao JDeng ZJiang MfalseEndogenous H<sub>2</sub>S-Triggered In Situ Synthesis of NIR-II-Emitting Nanoprobe for In Vivo Intelligently Lighting Up Colorectal Cancer.Overexpression of endogenous H<sub>2</sub>S is one of the key characteristic in colon cancer. However, developing endogenous H<sub>2</sub>S-activated optical probes for specific diagnosis of colorectal cancer is rarely explored. Herein, an in situ H<sub>2</sub>S-activatable second near-infrared (NIR-II)-emitting nanoprobe based on Ag-chicken egg white (Ag-CEW) complex for intelligently lighting up colorectal cancer was explored. The designed Ag-CEW complex holds efficient NIR-II emission of 1,000-1,400 nm via endogenous H<sub>2</sub>S-induced in situ chemical reaction to form Ag<sub>2</sub>S quantum dots (QDs). After reaction, the designed Ag-CEW complex with high photo-stability and biocompatibility was successfully used for NIR-II imaging-guided specific visualization and precise locatio2019-01-01T00:00:00Z2019 Jul2025-04-05T13:05:17.042Z2019-07-30T07:05:20ZS-EPMC66259703130163210.1016/j.isci.2019.06.034