biostudies-literatureKraitavin WMinistry of Education, Culture, Sports, Science and TechnologyE21https://www.ebi.ac.uk/biostudies/studies/S-EPMC6628215biostudies-literatureUnknown8(2)Understanding the mechanism of high-temperature tolerance in cold-freshwater fish is crucial for predicting how certain species will cope with global warming. In this study, we investigated temperature tolerance in masu salmon (<i>Oncorhynchus masou</i>, known in Japan as 'yamame'), an important aquaculture species. By selective breeding, we developed a group of yamame (F2) with high-temperature tolerance. This group was subjected to a high-temperature tolerance test and divided into two groups: High-temperature tolerant (HT) and non-high-temperature tolerant (NT). RNA was extracted from the gill and adipose fin tissues of each group, and the mRNA expression profiles were analyzed using RNA sequencing. A total of 2893 differentially expressed genes (DEGs) from the gill and 836 from the adiBiologyTranscriptome Analysis of Yamame (<i>Oncorhynchus masou</i>) in Normal Conditions after Heat Stress.PMC6628215Fiscal Year 2016Kambayashi DKraitavin WWatabe SIgarashi YMitsuyama SKinoshita SAsakawa SYoshitake KfalseTranscriptome Analysis of Yamame (<i>Oncorhynchus masou</i>) in Normal Conditions after Heat Stress.Understanding the mechanism of high-temperature tolerance in cold-freshwater fish is crucial for predicting how certain species will cope with global warming. In this study, we investigated temperature tolerance in masu salmon (<i>Oncorhynchus masou</i>, known in Japan as 'yamame'), an important aquaculture species. By selective breeding, we developed a group of yamame (F2) with high-temperature tolerance. This group was subjected to a high-temperature tolerance test and divided into two groups: High-temperature tolerant (HT) and non-high-temperature tolerant (NT). RNA was extracted from the gill and adipose fin tissues of each group, and the mRNA expression profiles were analyzed using RNA sequencing. A total of 2893 differentially expressed genes (DEGs) from the gill and 836 from the adi2019-01-01T00:00:00Z2019 Mar2026-05-07T07:54:30.898Z2019-07-30T07:06:03ZS-EPMC66282153093485110.3390/biology8020021