biostudies-literatureKraberger SBrian Mason Scientific and Technical Trust of New ZealandE532https://www.ebi.ac.uk/biostudies/studies/S-EPMC6630596biostudies-literatureUnknown11(6)Over the last decade, arthropods have been shown to harbour a rich diversity of viruses. Through viral metagenomics a large diversity of single-stranded (ss) DNA viruses have been identified. Here we examine the ssDNA virome of the hematophagous New Zealand blackfly using viral metagenomics. Our investigation reveals a plethora of novel ssDNA viral genomes, some of which cluster in the viral families <i>Genomoviridae</i> (<i>n</i> = 9), <i>Circoviridae</i> (<i>n</i> = 1), and <i>Microviridae</i> (<i>n</i> = 108), others in putative families that, at present, remain unclassified (<i>n</i> = 20) and one DNA molecule that only encodes a replication associated protein. Among these novel viruses, two putative multi-component virus genomes were recovered, and these are most closely related to a VirusesUnravelling the Single-Stranded DNA Virome of the New Zealand Blackfly.PMC6630596grant # 2015/09Fontenele RSWalters MKraberger SSchmidlin KVarsani AfalseUnravelling the Single-Stranded DNA Virome of the New Zealand Blackfly.Over the last decade, arthropods have been shown to harbour a rich diversity of viruses. Through viral metagenomics a large diversity of single-stranded (ss) DNA viruses have been identified. Here we examine the ssDNA virome of the hematophagous New Zealand blackfly using viral metagenomics. Our investigation reveals a plethora of novel ssDNA viral genomes, some of which cluster in the viral families <i>Genomoviridae</i> (<i>n</i> = 9), <i>Circoviridae</i> (<i>n</i> = 1), and <i>Microviridae</i> (<i>n</i> = 108), others in putative families that, at present, remain unclassified (<i>n</i> = 20) and one DNA molecule that only encodes a replication associated protein. Among these novel viruses, two putative multi-component virus genomes were recovered, and these are most closely related to a 2019-01-01T00:00:00Z2019 Jun2026-04-07T18:33:40.147Z2019-08-23T07:01:05ZS-EPMC66305963118173010.3390/v11060532