{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Macho AP"],"pubmed_abstract":["In recent years, many efforts have been directed towards the identification of new type III-secreted effectors, and the completion of the secretomes of several Pseudomonas syringae pathovars. Several functional and bioinformatic screenings have been used to search for candidates, which have been tested for translocation into the plant cell, an essential criterion for the identification of new type III effector proteins. The most common translocation assay is based on the use of DeltaAvrRpt2 as a reporter. When fused to a type III effector protein, DeltaAvrRpt2 is translocated and elicits a hypersensitive response in leaves of Arabidopsis thaliana expressing the RPS2 resistance protein. This approach has been used widely and has allowed the identification of a considerable number of new eff"],"journal":["Molecular plant pathology"],"pagination":["69-80"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6640233"],"repository":["biostudies-literature"],"pubmed_title":["Identification of new type III effectors and analysis of the plant response by competitive index."],"pmcid":["PMC6640233"],"pubmed_authors":["Ruiz-Albert J","Macho AP","Beuzon CR","Tornero P"],"additional_accession":[]},"is_claimable":false,"name":"Identification of new type III effectors and analysis of the plant response by competitive index.","description":"In recent years, many efforts have been directed towards the identification of new type III-secreted effectors, and the completion of the secretomes of several Pseudomonas syringae pathovars. Several functional and bioinformatic screenings have been used to search for candidates, which have been tested for translocation into the plant cell, an essential criterion for the identification of new type III effector proteins. The most common translocation assay is based on the use of DeltaAvrRpt2 as a reporter. When fused to a type III effector protein, DeltaAvrRpt2 is translocated and elicits a hypersensitive response in leaves of Arabidopsis thaliana expressing the RPS2 resistance protein. This approach has been used widely and has allowed the identification of a considerable number of new eff","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Jan","modification":"2025-05-29T20:30:31.967Z","creation":"2025-05-29T20:30:31.967Z"},"accession":"S-EPMC6640233","cross_references":{"pubmed":["19161354"],"doi":["10.1111/j.1364-3703.2008.00511.x"]}}