biostudies-literatureTabula Muris ConsortiumBLRD VANIDDK NIH HHSNIA NIH HHSRRD VANCI NIH HHSNLM NIH HHS367-372https://www.ebi.ac.uk/biostudies/studies/S-EPMC6642641biostudies-literatureUnknown562(7727)Here we present a compendium of single-cell transcriptomic data from the model organism Mus musculus that comprises more than 100,000 cells from 20 organs and tissues. These data represent a new resource for cell biology, reveal gene expression in poorly characterized cell populations and enable the direct and controlled comparison of gene expression in cell types that are shared between tissues, such as T lymphocytes and endothelial cells from different anatomical locations. Two distinct technical approaches were used for most organs: one approach, microfluidic droplet-based 3'-end counting, enabled the survey of thousands of cells at relatively low coverage, whereas the other, full-length transcript analysis based on fluorescence-activated cell sorting, enabled the characterization of cell types with high sensitivity and coverage. The cumulative data provide the foundation for an atlas of transcriptomic cell biology.NatureSingle-cell transcriptomics of 20 mouse organs creates a Tabula Muris.PMC6642641P30 DK026743DP1 AG053015I01 RX001222P30 DK116074I01 BX002324R01 CA157877K08 DK101603DP1 LM012179Travaglini KJZhang FKershner AMLehallier BNabhan ANGeorge BMWeinberg KCastro PWyss-Coray TTato CXue SPeng WCKumar MEMay APBaghel ASMetzger RJCirolia GLo ABeachy PAOverall coordinationNusse RChan CKFHang YIram TMignardi MLee DPLibrary preparation and sequencingEspinoza FHWu SMMcKay MHosseinzadeh SRando TAGulati GSSonnenburg JBotvinnik OTabula Muris ConsortiumBilen BCain CClarke MFNguyen PKIves FZhou LSupplemental text writing groupComputational data analysisKiss BMGreen FLee SEWang BMde Morree AMay OLPenland LNoh JDulgeroff LBTvan Weele LJMaynard ANg KMZanini Fdu Bois HQuake SRSit RVBansal IChen MBSzade KKarnam GBrownfield DEbadi HKong WTan SYPisco AOBakerman IYoungyunpipatkul JKrasnow MAWebber JTBerdnik DYousef HHuang KCChen SHuang AKim SKDarmanis SLi GSikandar SSCell type annotationBatson JWeissman ILKao KSLi QManjunath AZardeneta MEPrincipal investigatorsDemir KIsobe TWriting groupSinha ROrgan collection and processingTan WCroote DConley SDMin DJones RCBarres BASchaum NTropini CLiu LXiang JLam JYLu WJWaldburger LTellez KKuo CSFish MGillich ACho MWosczyna MNDeRisi JLGu XNeff NFRulifson EJPuccinelli RDivita TMay KLGenetiano GDemers ALogistical coordinationGan QPatkar RStanley GMKarkanias JfalseSingle-cell transcriptomics of 20 mouse organs creates a Tabula Muris.Here we present a compendium of single-cell transcriptomic data from the model organism Mus musculus that comprises more than 100,000 cells from 20 organs and tissues. These data represent a new resource for cell biology, reveal gene expression in poorly characterized cell populations and enable the direct and controlled comparison of gene expression in cell types that are shared between tissues, such as T lymphocytes and endothelial cells from different anatomical locations. Two distinct technical approaches were used for most organs: one approach, microfluidic droplet-based 3'-end counting, enabled the survey of thousands of cells at relatively low coverage, whereas the other, full-length transcript analysis based on fluorescence-activated cell sorting, enabled the characterization of cell types with high sensitivity and coverage. The cumulative data provide the foundation for an atlas of transcriptomic cell biology.2018-01-01T00:00:00Z2018 Oct2020-11-19T08:28:27Z2019-07-25T07:17:54ZS-EPMC66426413028314110.1038/s41586-018-0590-4