biostudies-literatureLebegue EH2020 Marie Sklodowska-Curie ActionsMinist?re de l&apos;Education Nationale, de l&apos;Enseignement Superieur et de la RechercheMinist?re de l?Europe et des Affaires ?trang?resFCT - Funda??o para a Ci?ncia e a Tecnologia9035-9042https://www.ebi.ac.uk/biostudies/studies/S-EPMC6644398biostudies-literatureUnknown3(8)The electrochemical systems of both grafted catechol as a pH-responsive electrophore and immobilized cytochrome <i>c</i> as a model redox protein are detected by cyclic voltammetry at an optimized lipid deposit-modified glassy carbon electrode. The catechol covalent grafting is successfully performed by the one-pot/three-step electrochemical reduction of 3,4-dihydroxybenzenediazonium salts generated in situ from 4-nitrocatechol. The resulting glassy carbon electrode electrochemically modified by grafted catechol species is evaluated as an efficient electrochemical pH sensor. The optimized molar ratio for the lipid deposit, promoting cytochrome <i>c</i> electrochemical activity in solution onto glassy carbon electrode, is reached for the lipid mixture composed of 75% 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphocholine and 25% cardiolipin. Cytochrome <i>c</i> immobilization into the optimized supported lipid deposit is efficiently achieved by cyclic voltammetry (10 cycles) recorded at the modified glassy carbon electrode in a cytochrome <i>c</i> solution. The pH-dependent redox response of the grafted catechol and that of the immobilized cytochrome <i>c</i> are finally detected at the same lipid-modified glassy carbon electrode without alteration of their structure and electrochemical properties in the pH range 5-9.ACS omegaElectrochemical Detection of pH-Responsive Grafted Catechol and Immobilized Cytochrome <i>c</i> onto Lipid Deposit-Modified Glassy Carbon Surface.PMC664439840814ZE745689Louro ROLebegue EBarriere FfalseElectrochemical Detection of pH-Responsive Grafted Catechol and Immobilized Cytochrome <i>c</i> onto Lipid Deposit-Modified Glassy Carbon Surface.The electrochemical systems of both grafted catechol as a pH-responsive electrophore and immobilized cytochrome <i>c</i> as a model redox protein are detected by cyclic voltammetry at an optimized lipid deposit-modified glassy carbon electrode. The catechol covalent grafting is successfully performed by the one-pot/three-step electrochemical reduction of 3,4-dihydroxybenzenediazonium salts generated in situ from 4-nitrocatechol. The resulting glassy carbon electrode electrochemically modified by grafted catechol species is evaluated as an efficient electrochemical pH sensor. The optimized molar ratio for the lipid deposit, promoting cytochrome <i>c</i> electrochemical activity in solution onto glassy carbon electrode, is reached for the lipid mixture composed of 75% 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphocholine and 25% cardiolipin. Cytochrome <i>c</i> immobilization into the optimized supported lipid deposit is efficiently achieved by cyclic voltammetry (10 cycles) recorded at the modified glassy carbon electrode in a cytochrome <i>c</i> solution. The pH-dependent redox response of the grafted catechol and that of the immobilized cytochrome <i>c</i> are finally detected at the same lipid-modified glassy carbon electrode without alteration of their structure and electrochemical properties in the pH range 5-9.2018-01-01T00:00:00Z2018 Aug2024-02-14T20:16:30.719Z2019-08-31T07:03:49ZS-EPMC66443983145903710.1021/acsomega.8b01425