{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li C"],"funding":["Natural Science Foundation of Shandong Province","NCI NIH HHS"],"pagination":["628-639"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6644699"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["79(6)"],"pubmed_abstract":["<h4>Background</h4>Alternol is a natural compound isolated from fermentation products of a mutant fungus. Our previous studies demonstrated that Alternol specifically kills cancer cells but spares benign cells.<h4>Methods</h4>To investigate the mechanism underlying alternol-induced cancer cell-specific killing effect, we took a comprehensive strategy to identify Alternol's protein targets in prostate cancer cells, including PC-3, C4-2, and 22RV1, plus benign BPH1 cell lines. Major experimental techniques included biotin-streptavidin pulldown assay coupled with mass-spectrometry, in vitro enzyme activity assay for Krebs cycle enzymes and gas chromatography-mass spectrometry (GC-MS) for metabolomic analysis.<h4>Results</h4>Among 14 verified protein targets, four were Krebs cycle enzymes, fum"],"journal":["The Prostate"],"pubmed_title":["Alternol eliminates excessive ATP production by disturbing Krebs cycle in prostate cancer."],"pmcid":["PMC6644699"],"funding_grant_id":["ZR2016HL25","R01 CA173292"],"pubmed_authors":["He C","Li C","Krishnamurthy P","Chavan H","Holzbeierlein JM","Xu H","Han S","Forrest ML","Duan S","Artigues A","Li B","Tang Y","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Alternol eliminates excessive ATP production by disturbing Krebs cycle in prostate cancer.","description":"<h4>Background</h4>Alternol is a natural compound isolated from fermentation products of a mutant fungus. Our previous studies demonstrated that Alternol specifically kills cancer cells but spares benign cells.<h4>Methods</h4>To investigate the mechanism underlying alternol-induced cancer cell-specific killing effect, we took a comprehensive strategy to identify Alternol's protein targets in prostate cancer cells, including PC-3, C4-2, and 22RV1, plus benign BPH1 cell lines. Major experimental techniques included biotin-streptavidin pulldown assay coupled with mass-spectrometry, in vitro enzyme activity assay for Krebs cycle enzymes and gas chromatography-mass spectrometry (GC-MS) for metabolomic analysis.<h4>Results</h4>Among 14 verified protein targets, four were Krebs cycle enzymes, fum","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 May","modification":"2026-05-04T23:37:41.279Z","creation":"2020-10-29T14:04:58Z"},"accession":"S-EPMC6644699","cross_references":{"pubmed":["30663084"],"doi":["10.1002/pros.23767"]}}