biostudies-literatureOu-Yang FMinistry of Science and Technology, TaiwanNational Sun Yat-sen University-KMU Joint Research ProjectKaohsiung Medical University HospitalE3238https://www.ebi.ac.uk/biostudies/studies/S-EPMC6651324biostudies-literatureUnknown20(13)Extracts from the Nepenthes plant have anti-microorganism and anti-inflammation effects. However, the anticancer effect of the Nepenthes plant is rarely reported, especially for breast cancer cells. Here, we evaluate the antitumor effects of the ethyl acetate extract of Nepenthesthorellii x (ventricosa x maxima) (EANT) against breast cancer cells. Cell viability and flow cytometric analyses were used to analyze apoptosis, oxidative stress, and DNA damage. EANT exhibits a higher antiproliferation ability to two breast cancer cell lines (MCF7 and SKBR3) as compared to normal breast cells (M10). A mechanistic study demonstrates that EANT induces apoptosis in breast cancer cells with evidence of subG1 accumulation and annexin V increment. EANT also induces glutathione (GSH) depletion, resultinInternational journal of molecular sciencesAntiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima).PMC6651324MOST 107-2314-B-037-048MOHW 108-TDU-B-212-124016KMUH107-7R74MOST 107-2320-B-037-016#NSYSUKMU 108-P001108-CCH-KMU-008MOST 107-2311-B-214-003Chang HWYu SYYen CYOu-Yang FTsai IHTang JYChen SRCheng YBKao JKFarooqi AAfalseAntiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima).Extracts from the Nepenthes plant have anti-microorganism and anti-inflammation effects. However, the anticancer effect of the Nepenthes plant is rarely reported, especially for breast cancer cells. Here, we evaluate the antitumor effects of the ethyl acetate extract of Nepenthesthorellii x (ventricosa x maxima) (EANT) against breast cancer cells. Cell viability and flow cytometric analyses were used to analyze apoptosis, oxidative stress, and DNA damage. EANT exhibits a higher antiproliferation ability to two breast cancer cell lines (MCF7 and SKBR3) as compared to normal breast cells (M10). A mechanistic study demonstrates that EANT induces apoptosis in breast cancer cells with evidence of subG1 accumulation and annexin V increment. EANT also induces glutathione (GSH) depletion, resultin2019-01-01T00:00:00Z2019 Jul2025-04-22T00:06:07.939Z2019-08-12T07:02:48ZS-EPMC66513243126622410.3390/ijms20133238