biostudies-literatureHa SMMinistry of Science and ICTMinistry of Health &amp; Welfare, KoreaKorea Health Industry Development InstituteBioNano Health Guard Research CenterMinistry of Science, ICT and Future PlanningNational Research Foundation of Korea530-536https://www.ebi.ac.uk/biostudies/studies/S-EPMC6660342biostudies-literatureUnknown39(6)<h4>Background</h4>Next-generation sequencing is increasingly used for taxonomic identification of pathogenic bacterial isolates. We evaluated the performance of a newly introduced whole genome-based bacterial identification system, TrueBac ID (ChunLab Inc., Seoul, Korea), using clinical isolates that were not identified by three matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems and 16S rRNA gene sequencing.<h4>Methods</h4>Thirty-six bacterial isolates were selected from a university-affiliated hospital and a commercial clinical laboratory. Species was identified by three MALDI-TOF MS systems: Bruker Biotyper MS (Bruker Daltonics, Billerica, MA, USA), VITEK MS (bioMérieux, Marcy l'Étoile, France), and ASTA MicroIDSys (ASTA Inc., Suwon, KoreAnnals of laboratory medicineApplication of the Whole Genome-Based Bacterial Identification System, TrueBac ID, Using Clinical Isolates That Were Not Identified With Three Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems.PMC6660342H-GUARD_2014M3A6B2060509HI17C18072017M3A7B4039936Ha SMRoh JChoi SBByun JHYang SJKim CKYong DChun JfalseApplication of the Whole Genome-Based Bacterial Identification System, TrueBac ID, Using Clinical Isolates That Were Not Identified With Three Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems.<h4>Background</h4>Next-generation sequencing is increasingly used for taxonomic identification of pathogenic bacterial isolates. We evaluated the performance of a newly introduced whole genome-based bacterial identification system, TrueBac ID (ChunLab Inc., Seoul, Korea), using clinical isolates that were not identified by three matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems and 16S rRNA gene sequencing.<h4>Methods</h4>Thirty-six bacterial isolates were selected from a university-affiliated hospital and a commercial clinical laboratory. Species was identified by three MALDI-TOF MS systems: Bruker Biotyper MS (Bruker Daltonics, Billerica, MA, USA), VITEK MS (bioMérieux, Marcy l'Étoile, France), and ASTA MicroIDSys (ASTA Inc., Suwon, Kore2019-01-01T00:00:00Z2019 Nov2025-04-05T09:36:10.138Z2019-11-08T08:04:12ZS-EPMC66603423124088010.3343/alm.2019.39.6.530