{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Richardson SC"],"funding":["Wellcome Trust","NIGMS NIH HHS","Engineering and Physical Sciences Research Council"],"pagination":["1-11"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6661902"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["127(1)"],"pubmed_abstract":["Macromolecular therapeutics and nano-sized drug delivery systems often require localisation to specific intracellular compartments. In particular, efficient endosomal escape, retrograde trafficking, or late endocytic/lysosomal activation are often prerequisites for pharmacological activity. The aim of this study was to define a fluorescence microscopy technique able to confirm the localisation of water-soluble polymeric carriers to late endocytic intracellular compartments. Three polymeric carriers of different molecular weight and character were studied: dextrin (Mw~50,000 g/mol), a N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer (Mw approximately 35,000 g/mol) and polyethylene glycol (PEG) (Mw 5000 g/mol). They were labelled with Oregon Green (OG) (0.3-3 wt.%; <3% free OG in respect o"],"journal":["Journal of controlled release : official journal of the Controlled Release Society"],"pubmed_title":["The use of fluorescence microscopy to define polymer localisation to the late endocytic compartments in cells that are targets for drug delivery."],"pmcid":["PMC6661902"],"funding_grant_id":["R01 GM058202-11","R01 GM058202","EP/C013220/1"],"pubmed_authors":["Davies MW","Piper RC","Deacon SP","Wallom KL","Powell AJ","Duncan R","Ferguson EL","Richardson SC"],"additional_accession":[]},"is_claimable":false,"name":"The use of fluorescence microscopy to define polymer localisation to the late endocytic compartments in cells that are targets for drug delivery.","description":"Macromolecular therapeutics and nano-sized drug delivery systems often require localisation to specific intracellular compartments. In particular, efficient endosomal escape, retrograde trafficking, or late endocytic/lysosomal activation are often prerequisites for pharmacological activity. The aim of this study was to define a fluorescence microscopy technique able to confirm the localisation of water-soluble polymeric carriers to late endocytic intracellular compartments. Three polymeric carriers of different molecular weight and character were studied: dextrin (Mw~50,000 g/mol), a N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer (Mw approximately 35,000 g/mol) and polyethylene glycol (PEG) (Mw 5000 g/mol). They were labelled with Oregon Green (OG) (0.3-3 wt.%; <3% free OG in respect o","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Apr","modification":"2025-04-22T15:09:33.472Z","creation":"2019-08-02T07:02:38Z"},"accession":"S-EPMC6661902","cross_references":{"pubmed":["18281120"],"doi":["10.1016/j.jconrel.2007.12.015"]}}