{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bromfield ESP"],"funding":["Agriculture and Agri-Food"],"pagination":["6999-7004"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6662265"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(12)"],"pubmed_abstract":["The toxic legume plant, <i>Galega officinalis,</i> is native to the Eastern Mediterranean and Black Sea regions. This legume is considered to be a noxious weed, and its establishment in Canada may have resulted from ornamental planting and/or field trials. In its native range, a highly specific nitrogen-fixing symbiosis with the bacterium, <i>Neorhizobium galegae</i> symbiovar (sv.) officinalis, is required for normal growth. In North America, nothing is known about the bacterial symbionts of <i>G. officinalis</i>. Our purpose was to determine the species and symbiovar identity of symbiotic bacteria associated with invasive plants of <i>G. officinalis</i> at five sites in the province of Ontario, Canada.  Sequence analysis of four housekeeping (16S rRNA, <i>atp</i>D, <i>gln</i>II, and <i>rec</i>A) and two symbiosis (<i>nod</i>C and <i>nif</i>H) genes showed that all 50 bacterial isolates from root nodules of <i>G. officinalis</i> at the five Canadian sites were identical to strains of <i>N. galegae</i> sv. officinalis originating either from Europe or the Caucasus. Plant tests indicated that soils collected from four Canadian sites without a history of agriculture or presence of <i>G. officinalis</i> were deficient in symbiotic bacteria capable of eliciting nodules on this plant. Collectively our data support the hypothesis of anthropogenic co-introduction of <i>G. officinalis</i> and its specific symbiotic bacterium into Canada from the Old World. Factors that may limit the spread of <i>G. officinalis</i> in new environments are discussed."],"journal":["Ecology and evolution"],"pubmed_title":["Invasive <i>Galega officinalis</i> (Goat's rue) plants in Canada form a symbiotic association with strains of <i>Neorhizobium galegae</i> sv. officinalis originating from the Old World."],"pmcid":["PMC6662265"],"funding_grant_id":["1666","1318"],"pubmed_authors":["Tran Thi TV","Darbyshire SJ","Cloutier S","Robidas C","Bromfield ESP"],"additional_accession":[]},"is_claimable":false,"name":"Invasive <i>Galega officinalis</i> (Goat's rue) plants in Canada form a symbiotic association with strains of <i>Neorhizobium galegae</i> sv. officinalis originating from the Old World.","description":"The toxic legume plant, <i>Galega officinalis,</i> is native to the Eastern Mediterranean and Black Sea regions. This legume is considered to be a noxious weed, and its establishment in Canada may have resulted from ornamental planting and/or field trials. In its native range, a highly specific nitrogen-fixing symbiosis with the bacterium, <i>Neorhizobium galegae</i> symbiovar (sv.) officinalis, is required for normal growth. In North America, nothing is known about the bacterial symbionts of <i>G. officinalis</i>. Our purpose was to determine the species and symbiovar identity of symbiotic bacteria associated with invasive plants of <i>G. officinalis</i> at five sites in the province of Ontario, Canada.  Sequence analysis of four housekeeping (16S rRNA, <i>atp</i>D, <i>gln</i>II, and <i>rec</i>A) and two symbiosis (<i>nod</i>C and <i>nif</i>H) genes showed that all 50 bacterial isolates from root nodules of <i>G. officinalis</i> at the five Canadian sites were identical to strains of <i>N. galegae</i> sv. officinalis originating either from Europe or the Caucasus. Plant tests indicated that soils collected from four Canadian sites without a history of agriculture or presence of <i>G. officinalis</i> were deficient in symbiotic bacteria capable of eliciting nodules on this plant. Collectively our data support the hypothesis of anthropogenic co-introduction of <i>G. officinalis</i> and its specific symbiotic bacterium into Canada from the Old World. Factors that may limit the spread of <i>G. officinalis</i> in new environments are discussed.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2024-12-04T04:43:46.226Z","creation":"2019-08-07T07:07:48Z"},"accession":"S-EPMC6662265","cross_references":{"pubmed":["31380028"],"doi":["10.1002/ece3.5266"]}}