biostudies-literatureFuche FJNIAID NIH HHS1427-1435https://www.ebi.ac.uk/biostudies/studies/S-EPMC6663134biostudies-literatureUnknown15(6)Non-typhoidal <i>Salmonella</i> (NTS) are a leading cause of foodborne infections worldwide, and serogroups B, C₁, C_2-C₃ and D are the most common serogroups associated with human disease. While live vaccine candidates that protect against <i>S.</i> Typhimurium (serogroup B) and <i>S.</i> Enteritidis (serogroup D) have been described by us and others, far less effort has been directed towards vaccines that target either serogroup C₁ or C_2-C₃ <i>Salmonella</i>. Here we describe a <i>Salmonella</i> Newport-based live-attenuated vaccine (serogroup C_2-C₃). Deletion of the genes <i>clpX</i> or <i>rfaL</i>, previously used in live vaccines to attenuate <i>S.</i> Typhimurium and/or <i>S.</i> Enteritidis, faileHuman vaccines & immunotherapeuticsDeletions in <i>guaBA</i> and <i>htrA</i> but not <i>clpX</i> or <i>rfaL</i> constitute a live-attenuated vaccine strain of <i>Salmonella</i> Newport to protect against serogroup C_2-C₃ <i>Salmonella</i> in mice.PMC6663134U19 AI109776Fuche FJRamachandran GJones JATennant SMHigginson EESimon RfalseDeletions in <i>guaBA</i> and <i>htrA</i> but not <i>clpX</i> or <i>rfaL</i> constitute a live-attenuated vaccine strain of <i>Salmonella</i> Newport to protect against serogroup C_2-C₃ <i>Salmonella</i> in mice.Non-typhoidal <i>Salmonella</i> (NTS) are a leading cause of foodborne infections worldwide, and serogroups B, C₁, C_2-C₃ and D are the most common serogroups associated with human disease. While live vaccine candidates that protect against <i>S.</i> Typhimurium (serogroup B) and <i>S.</i> Enteritidis (serogroup D) have been described by us and others, far less effort has been directed towards vaccines that target either serogroup C₁ or C_2-C₃ <i>Salmonella</i>. Here we describe a <i>Salmonella</i> Newport-based live-attenuated vaccine (serogroup C_2-C₃). Deletion of the genes <i>clpX</i> or <i>rfaL</i>, previously used in live vaccines to attenuate <i>S.</i> Typhimurium and/or <i>S.</i> Enteritidis, faile2019-01-01T00:00:00Z20192025-04-04T11:43:12.057Z2019-08-09T07:03:55ZS-EPMC66631342992772510.1080/21645515.2018.1491499