{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li Y"],"funding":["NIDDK NIH HHS","NHLBI NIH HHS","HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI)","Barth Syndrome Foundation (BSF)"],"pagination":["11568-11578"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6663869"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["294(30)"],"pubmed_abstract":["Cardiolipin (CL) is the signature phospholipid of mitochondrial membranes. Although it has long been known that CL plays an important role in mitochondrial bioenergetics, recent evidence in the yeast model indicates that CL is also essential for intermediary metabolism. To gain insight into the function of CL in energy metabolism in mammalian cells, here we analyzed the metabolic flux of [U-<sup>13</sup>C]glucose in a mouse C2C12 myoblast cell line, TAZ-KO, which is CL-deficient because of CRISPR/Cas9-mediated knockout of the CL-remodeling enzyme tafazzin (TAZ). TAZ-KO cells exhibited decreased flux of [U-<sup>13</sup>C]glucose to [<sup>13</sup>C]acetyl-CoA and M2 and M4 isotopomers of tricarboxylic acid (TCA) cycle intermediates. The activity of pyruvate carboxylase, the predominant enzym"],"journal":["The Journal of biological chemistry"],"pubmed_title":["Cardiolipin-induced activation of pyruvate dehydrogenase links mitochondrial lipid biosynthesis to TCA cycle function."],"pmcid":["PMC6663869"],"funding_grant_id":["HL117880","R01 HL117880","U24 DK097153"],"pubmed_authors":["Li Y","Reynolds CA","Schlame M","Raja V","Yu W","Lou W","Denis S","Schmidtke MW","Greenberg ML","Houtkooper RH"],"additional_accession":[]},"is_claimable":false,"name":"Cardiolipin-induced activation of pyruvate dehydrogenase links mitochondrial lipid biosynthesis to TCA cycle function.","description":"Cardiolipin (CL) is the signature phospholipid of mitochondrial membranes. Although it has long been known that CL plays an important role in mitochondrial bioenergetics, recent evidence in the yeast model indicates that CL is also essential for intermediary metabolism. To gain insight into the function of CL in energy metabolism in mammalian cells, here we analyzed the metabolic flux of [U-<sup>13</sup>C]glucose in a mouse C2C12 myoblast cell line, TAZ-KO, which is CL-deficient because of CRISPR/Cas9-mediated knockout of the CL-remodeling enzyme tafazzin (TAZ). TAZ-KO cells exhibited decreased flux of [U-<sup>13</sup>C]glucose to [<sup>13</sup>C]acetyl-CoA and M2 and M4 isotopomers of tricarboxylic acid (TCA) cycle intermediates. The activity of pyruvate carboxylase, the predominant enzym","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jul","modification":"2025-04-21T20:16:24.543Z","creation":"2025-04-05T17:53:17.04Z"},"accession":"S-EPMC6663869","cross_references":{"pubmed":["31186346"],"doi":["10.1074/jbc.ra119.009037","10.1074/jbc.RA119.009037"]}}