{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jeppesen DK"],"funding":["NIBIB NIH HHS","NIDDK NIH HHS","NHLBI NIH HHS","Vanderbilt Digestive Diseases Research Center","NCI NIH HHS","NIH","Vanderbilt-Ingram Cancer Center","NIGMS NIH HHS"],"pagination":["428-445.e18"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6664447"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["177(2)"],"pubmed_abstract":["The heterogeneity of small extracellular vesicles and presence of non-vesicular extracellular matter have led to debate about contents and functional properties of exosomes. Here, we employ high-resolution density gradient fractionation and direct immunoaffinity capture to precisely characterize the RNA, DNA, and protein constituents of exosomes and other non-vesicle material. Extracellular RNA, RNA-binding proteins, and other cellular proteins are differentially expressed in exosomes and non-vesicle compartments. Argonaute 1-4, glycolytic enzymes, and cytoskeletal proteins were not detected in exosomes. We identify annexin A1 as a specific marker for microvesicles that are shed directly from the plasma membrane. We further show that small extracellular vesicles are not vehicles of active "],"journal":["Cell"],"pubmed_title":["Reassessment of Exosome Composition."],"pmcid":["PMC6664447"],"funding_grant_id":["UG3 CA241685","P30 CA068485","F31 HL136081","R35 GM125028","P30 DK058404","P50 CA236733","U01-EB021214","U01 EB021214","U19 CA179514","R35 CA197570"],"pubmed_authors":["Ping J","Coffey RJ","Patton JG","Liebler DC","Jeppesen DK","Liu Q","Zhang Q","Franklin JL","Zimmerman LJ","Rome LH","Higginbotham JN","Evans R","Fenix AM","Fissell WH","Burnette DT"],"additional_accession":[]},"is_claimable":false,"name":"Reassessment of Exosome Composition.","description":"The heterogeneity of small extracellular vesicles and presence of non-vesicular extracellular matter have led to debate about contents and functional properties of exosomes. Here, we employ high-resolution density gradient fractionation and direct immunoaffinity capture to precisely characterize the RNA, DNA, and protein constituents of exosomes and other non-vesicle material. Extracellular RNA, RNA-binding proteins, and other cellular proteins are differentially expressed in exosomes and non-vesicle compartments. Argonaute 1-4, glycolytic enzymes, and cytoskeletal proteins were not detected in exosomes. We identify annexin A1 as a specific marker for microvesicles that are shed directly from the plasma membrane. We further show that small extracellular vesicles are not vehicles of active ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Apr","modification":"2026-05-04T03:39:20.112Z","creation":"2020-10-30T08:20:23Z"},"accession":"S-EPMC6664447","cross_references":{"pubmed":["30951670"],"doi":["10.1016/j.cell.2019.02.029"]}}