biostudies-literatureWang JNingxia High School first-class DisciplinesNational Natural Science Foundation of ChinaThe Key Research and Development Program of Ningxia Hui Autonomous Region227https://www.ebi.ac.uk/biostudies/studies/S-EPMC6664773biostudies-literatureUnknown10(1)<h4>Background</h4>High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still unknown because SSCs are rare in the testes.<h4>Methods</h4>In the present study, we first used in vitro-cultured SSCs to study the effect of heat shock treatment on SSC development. Then, we used RNA-Seq analysis to identify new genes or signalling pathways implicated in the heat stress response.<h4>Results</h4>We found that 45 min of 43 °C heat shock treatment significantly inhibited the proliferation of SSCs 2 h after treatment but did not lead to apoptosis. In total, 17,822 genes were identified by RNA-Seq aStem cell research & therapyHigh temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis.PMC66647732016KJHM4381460488NXYLXK2017B0781860266Liu XDeng SLMa WZJia HWang JGao WJfalseHigh temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis.<h4>Background</h4>High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still unknown because SSCs are rare in the testes.<h4>Methods</h4>In the present study, we first used in vitro-cultured SSCs to study the effect of heat shock treatment on SSC development. Then, we used RNA-Seq analysis to identify new genes or signalling pathways implicated in the heat stress response.<h4>Results</h4>We found that 45 min of 43 °C heat shock treatment significantly inhibited the proliferation of SSCs 2 h after treatment but did not lead to apoptosis. In total, 17,822 genes were identified by RNA-Seq a2019-01-01T00:00:00Z2019 Jul2025-04-05T10:21:51.308Z2019-08-09T07:04:06ZS-EPMC66647733135805910.1186/s13287-019-1335-5