biostudies-literatureMusa HMinistry of Higher Education314https://www.ebi.ac.uk/biostudies/studies/S-EPMC6667522biostudies-literatureUnknown9(8)Initially, a new moderate halophilic strain was locally isolated from seawater. The partial 16S rRNA sequence analysis positioned the organism in <i>Marinobacter</i> genus and was named '<i>Marinobacter litoralis</i> SW-45'. This study further demonstrates successful utilization of the halophilic <i>M. litoralis</i> SW-45 lipase (MLL) for butyl ester synthesis from crude palm fruit oil (CPO) and kernel oil (CPKO) in heptane and solvent-free system, respectively, using hydroesterification. Hydrolysis and esterification of enzymatic [<i>Thermomyces lanuginosus</i> lipase (TLL)] hydrolysis of CPO and CPKO to free fatty acids (FFA) followed by MLL-catalytic esterification of the concentrated FFAs with butanol (acyl acceptor) to synthesize butyl esters were performed. A one-factor-at-a-time tec3 BiotechBiosynthesis of butyl esters from crude oil of palm fruit and kernel using halophilic lipase secretion by <i>Marinobacter litoralis</i> SW-45.PMC66675229017-00007Gopinath SCBAhmad MAMusa HGunny AANKasim FHfalseBiosynthesis of butyl esters from crude oil of palm fruit and kernel using halophilic lipase secretion by <i>Marinobacter litoralis</i> SW-45.Initially, a new moderate halophilic strain was locally isolated from seawater. The partial 16S rRNA sequence analysis positioned the organism in <i>Marinobacter</i> genus and was named '<i>Marinobacter litoralis</i> SW-45'. This study further demonstrates successful utilization of the halophilic <i>M. litoralis</i> SW-45 lipase (MLL) for butyl ester synthesis from crude palm fruit oil (CPO) and kernel oil (CPKO) in heptane and solvent-free system, respectively, using hydroesterification. Hydrolysis and esterification of enzymatic [<i>Thermomyces lanuginosus</i> lipase (TLL)] hydrolysis of CPO and CPKO to free fatty acids (FFA) followed by MLL-catalytic esterification of the concentrated FFAs with butanol (acyl acceptor) to synthesize butyl esters were performed. A one-factor-at-a-time tec2019-01-01T00:00:00Z2019 Aug2025-06-01T12:03:13.508Z2025-06-01T12:03:13.508ZS-EPMC66675223140663610.1007/s13205-019-1845-y