biostudies-literatureRaijmakers RPQ-support Foundationofz296https://www.ebi.ac.uk/biostudies/studies/S-EPMC6667718biostudies-literatureUnknown6(7)Although most patients recover from acute Q fever, around 20% develop Q fever fatigue syndrome (QFS), a debilitating fatigue syndrome that lasts at least 6 months. This study investigated transcriptional profiles of circulating monocytes and circulating cytokines as a subsequent mirror of myeloid cell function, 1 and 6 months after an acute Q fever infection. Total RNA of circulating monocytes was collected from 11 acute Q fever patients and 15 healthy controls, matched for age (±5 years) and sex. Samples were collected at a median of 27 days (baseline, interquartile range, 15-35 days) after the infection and again 6 months thereafter. Transcriptome analysis was performed using RNA sequencing. Additionally, concentrations of circulating interleukin (IL)-10, IL-1β, IL-1Ra, and IL-6 were meaOpen forum infectious diseasesLong-Lasting Transcriptional Changes in Circulating Monocytes of Acute Q Fever Patients.PMC6667718UMCN-160708-00Keijmel SPBleeker-Rovers CPNguyen CTer Horst RStenos JJoosten LAVan Der Meer JWNovakovic BGraves SRRaijmakers RPNetea MGfalseLong-Lasting Transcriptional Changes in Circulating Monocytes of Acute Q Fever Patients.Although most patients recover from acute Q fever, around 20% develop Q fever fatigue syndrome (QFS), a debilitating fatigue syndrome that lasts at least 6 months. This study investigated transcriptional profiles of circulating monocytes and circulating cytokines as a subsequent mirror of myeloid cell function, 1 and 6 months after an acute Q fever infection. Total RNA of circulating monocytes was collected from 11 acute Q fever patients and 15 healthy controls, matched for age (±5 years) and sex. Samples were collected at a median of 27 days (baseline, interquartile range, 15-35 days) after the infection and again 6 months thereafter. Transcriptome analysis was performed using RNA sequencing. Additionally, concentrations of circulating interleukin (IL)-10, IL-1β, IL-1Ra, and IL-6 were mea2019-01-01T00:00:00Z2019 Jul2025-04-04T22:09:07.451Z2019-08-09T07:04:10ZS-EPMC66677183136377310.1093/ofid/ofz296