{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ooms M"],"funding":["Intramural NIH HHS","NCRR NIH HHS","NIMH NIH HHS"],"pagination":["1306-1313"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6668514"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(7)"],"pubmed_abstract":["Although still a matter of controversy, disrupted in schizophrenia protein 1 (DISC1) was suggested as a potential inhibitor of phosphodiesterase 4 (PDE4). We used Disc1 locus impairment (LI) mice to investigate the interaction between PDE4 and DISC 1 in vivo and in vitro. [11C](R)-Rolipram binding was measured by PET in LI (n = 11) and C57BL/6 wild-type (WT, n = 9) mice. [11C](R)-Rolipram total distribution volumes (VT) were calculated and corrected for plasma-free fraction (fP) measured in a separate group of LI (n = 6) and WT (n = 7) mice. PDE4 enzyme activity was measured using in vitro samples of cerebral cortices from groups of LI (n = 4), heterozygote (n = 4), and WT (n = 4) mice. Disc1 LI mice showed a 41% increase in VT (18 ± 6 vs. 13±4 mL/cm3, P = 0.04) compared to WT mice. VT/fP "],"journal":["Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism"],"pubmed_title":["[11C](R)-Rolipram positron emission tomography detects DISC1 inhibition of phosphodiesterase type 4 in live Disc1 locus-impaired mice."],"pmcid":["PMC6668514"],"funding_grant_id":["ZIA MH002795","P50 MH094268","ZIA MH002793","R01 MH105660","P41 RR001192"],"pubmed_authors":["Lohith TG","Jaaro-Peled H","Mabins SN","Kimura Y","Fujita M","Zoghbi SS","Innis RB","Sawa A","Ooms M","Tomoda T","Telu S","Tsujikawa T","Sumitomo A","Pike VW"],"additional_accession":[]},"is_claimable":false,"name":"[11C](R)-Rolipram positron emission tomography detects DISC1 inhibition of phosphodiesterase type 4 in live Disc1 locus-impaired mice.","description":"Although still a matter of controversy, disrupted in schizophrenia protein 1 (DISC1) was suggested as a potential inhibitor of phosphodiesterase 4 (PDE4). We used Disc1 locus impairment (LI) mice to investigate the interaction between PDE4 and DISC 1 in vivo and in vitro. [11C](R)-Rolipram binding was measured by PET in LI (n = 11) and C57BL/6 wild-type (WT, n = 9) mice. [11C](R)-Rolipram total distribution volumes (VT) were calculated and corrected for plasma-free fraction (fP) measured in a separate group of LI (n = 6) and WT (n = 7) mice. PDE4 enzyme activity was measured using in vitro samples of cerebral cortices from groups of LI (n = 4), heterozygote (n = 4), and WT (n = 4) mice. Disc1 LI mice showed a 41% increase in VT (18 ± 6 vs. 13±4 mL/cm3, P = 0.04) compared to WT mice. VT/fP ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jul","modification":"2025-04-04T13:33:05.552Z","creation":"2025-04-04T13:33:05.552Z"},"accession":"S-EPMC6668514","cross_references":{"pubmed":["29430995"],"doi":["10.1177/0271678x18758997","10.1177/0271678X18758997"]}}