{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["60(8)"],"submitter":["Rohrig W"],"funding":["Friedrich-Alexander-Universität Erlangen-Nürnberg"],"pubmed_abstract":["Endocannabinoids and endocannabinoid-related compounds (ERCs) are involved in many physiological processes. They are released on demand from phosphoinositide and <i>N</i>-acylphosphatidyl ethanolamine (NAPE) precursors and comprise 2-monoacylglycerols (2-MGs) and FA ethanolamides (FEAs). Despite the abundance of advanced quantitative methods, however, their determined concentrations in blood plasma are inconsistent because 2-MGs and FEAs undergo artifactual de novo formation, chemical isomerization, and degradation during sample collection and storage. For a comprehensive survey of these compounds in blood and plasma, we have developed and validated an ultra-HPLC-MS/MS method to quantify 24 endocannabinoids, ERCs, and their phospholipid precursors. Immediate acidification of EDTA-blood to "],"journal":["Journal of lipid research"],"pagination":["1475-1488"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6672038"],"repository":["biostudies-literature"],"pubmed_title":["Quantification of 24 circulating endocannabinoids, endocannabinoid-related compounds, and their phospholipid precursors in human plasma by UHPLC-MS/MS."],"pmcid":["PMC6672038"],"pubmed_authors":["Deutsch B","Rohrig W","Achenbach S","Pischetsrieder M"],"additional_accession":[]},"is_claimable":false,"name":"Quantification of 24 circulating endocannabinoids, endocannabinoid-related compounds, and their phospholipid precursors in human plasma by UHPLC-MS/MS.","description":"Endocannabinoids and endocannabinoid-related compounds (ERCs) are involved in many physiological processes. They are released on demand from phosphoinositide and <i>N</i>-acylphosphatidyl ethanolamine (NAPE) precursors and comprise 2-monoacylglycerols (2-MGs) and FA ethanolamides (FEAs). Despite the abundance of advanced quantitative methods, however, their determined concentrations in blood plasma are inconsistent because 2-MGs and FEAs undergo artifactual de novo formation, chemical isomerization, and degradation during sample collection and storage. For a comprehensive survey of these compounds in blood and plasma, we have developed and validated an ultra-HPLC-MS/MS method to quantify 24 endocannabinoids, ERCs, and their phospholipid precursors. Immediate acidification of EDTA-blood to ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Aug","modification":"2025-04-04T23:21:52.181Z","creation":"2025-04-04T23:21:52.181Z"},"accession":"S-EPMC6672038","cross_references":{"pubmed":["31235475"],"doi":["10.1194/jlr.d094680","10.1194/jlr.D094680"]}}