biostudies-literatureTimmons JAMedical Research CouncilNational Institute for Health Research (NIHR)152https://www.ebi.ac.uk/biostudies/studies/S-EPMC6676519biostudies-literatureUnknown20(1)Jacob and Speed did not identify even a single example of a '150-gene-set' that was statistically significant at classifying Alzheimer's disease (AD) samples, or age in independent studies. We attempt to clarify the various misunderstandings, below.Genome biologyA statistical and biological response to an informatics appraisal of healthy aging gene signatures.PMC6676519G1100015NF-SI-0616-10042Howard RTimmons JASood SCrossland HPhillips BGallagher IJAtherton PJKraus WEfalseA statistical and biological response to an informatics appraisal of healthy aging gene signatures.Jacob and Speed did not identify even a single example of a '150-gene-set' that was statistically significant at classifying Alzheimer's disease (AD) samples, or age in independent studies. We attempt to clarify the various misunderstandings, below.2019-01-01T00:00:00Z2019 Aug2026-05-07T01:46:25.972Z2026-04-07T22:28:05.767ZS-EPMC66765193137514710.1186/s13059-019-1734-z