biostudies-literatureWong TWMinistry of Science and Technology, TaiwanNational Cheng Kung UniversityNational Cheng Kung University HospitalCenter of Applied Nanomedicine, National Cheng Kung University from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in TaiwanChinese University of Hong KongFocused Investment Scheme and Li Ka Shing Institute of Health Science of the Chinese University of Hong KongE1028https://www.ebi.ac.uk/biostudies/studies/S-EPMC6678880biostudies-literatureUnknown8(7)Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profiJournal of clinical medicineProgenitor Cells Derived from Drain Waste Product of Open-Heart Surgery in Children.PMC6678880101-2314-B-6-031, 102-2314-B-006-018, 103-2314-B-006-077to H.C.C.B106-K028, B107-K048101-2314-B—6-031, 102-2314-B-006-018, 103-2314-B-006-07710705004 to C.D.K.972314B006018MY2, 106-2218-E-006-02010703019105-2628-B-006-003-MY3 to W.T.C.1002627E033001, 1072321B006008466710 to X.J.10506007 to J.N.W.to T.W.W.Lin HCWang JNKao CCChen JLee YPLin CWChan HCKan CDChou CHRuan YCWong TWJiang XYu CKChen IYTsao SChiu WTFok KLfalseProgenitor Cells Derived from Drain Waste Product of Open-Heart Surgery in Children.Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profi2019-01-01T00:00:00Z2019 Jul2026-05-07T06:42:29.231Z2019-08-23T07:02:36ZS-EPMC66788803133692710.3390/jcm8071028