{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":52,"searchCount":0},"additional":{"submitter":["Sato K"],"funding":["Hyogo Science and Technology Association Grant","Takeda Science Foundation","Ministry of Education, Culture, Sports, Science and Technology"],"pagination":["e1008309"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6690551"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(7)"],"pubmed_abstract":["Males of the Drosophila melanogaster mutant croaker (cro) generate a polycyclic pulse song dissimilar to the monocyclic songs typical of wild-type males during courtship. However, cro has not been molecularly mapped to any gene in the genome. We demonstrate that cro is a mutation in the gene encoding the Calmodulin-binding transcription factor (Camta) by genetic complementation tests with chromosomal deficiencies, molecular cloning of genomic fragments that flank the cro-mutagenic P-insertion, and phenotypic rescue of the cro mutant phenotype by Camta+-encoding cDNA as well as a BAC clone containing the gene for Camta. We further show that knockdown of the Camta-encoding gene phenocopies cro mutant songs when targeted to a subset of fruitless-positive neurons that include the mcALa and AL1 clusters in the brain. cro-GAL4 and an anti-Camta antibody labeled a large number of brain neurons including mcALa. We conclude that the Camta-encoding gene represents the cro locus, which has been implicated in a species-specific difference in courtship songs between D. sechellia and simulans."],"journal":["PLoS genetics"],"pubmed_title":["Calmodulin-binding transcription factor shapes the male courtship song in Drosophila."],"pmcid":["PMC6690551"],"funding_grant_id":["19H04923","16H06371","156522","19H04766","17K07040"],"pubmed_authors":["Koganezawa M","Ote M","Ahsan MT","Yamamoto D","Sato K"],"view_count":["52"],"additional_accession":[]},"is_claimable":false,"name":"Calmodulin-binding transcription factor shapes the male courtship song in Drosophila.","description":"Males of the Drosophila melanogaster mutant croaker (cro) generate a polycyclic pulse song dissimilar to the monocyclic songs typical of wild-type males during courtship. However, cro has not been molecularly mapped to any gene in the genome. We demonstrate that cro is a mutation in the gene encoding the Calmodulin-binding transcription factor (Camta) by genetic complementation tests with chromosomal deficiencies, molecular cloning of genomic fragments that flank the cro-mutagenic P-insertion, and phenotypic rescue of the cro mutant phenotype by Camta+-encoding cDNA as well as a BAC clone containing the gene for Camta. We further show that knockdown of the Camta-encoding gene phenocopies cro mutant songs when targeted to a subset of fruitless-positive neurons that include the mcALa and AL1 clusters in the brain. cro-GAL4 and an anti-Camta antibody labeled a large number of brain neurons including mcALa. We conclude that the Camta-encoding gene represents the cro locus, which has been implicated in a species-specific difference in courtship songs between D. sechellia and simulans.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jul","modification":"2024-11-15T16:40:06.766Z","creation":"2019-08-20T07:04:48Z"},"accession":"S-EPMC6690551","cross_references":{"pubmed":["31344027"],"doi":["10.1371/journal.pgen.1008309"]}}