{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sugiura K"],"funding":["MEXT | Japan Society for the Promotion of Science (JSPS)"],"pagination":["12091-12098"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6690705"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["294(32)"],"pubmed_abstract":["Thiol-based redox regulation via ferredoxin-thioredoxin (Trx) reductase/Trx controls various functions in chloroplasts in response to light/dark changes. Trx is a key factor of this regulatory system, and five Trx subtypes, including 10 isoforms, have been identified as chloroplast-localized forms in <i>Arabidopsis thaliana</i> These subtypes display distinct target selectivity, and, consequently, they form a complicated redox regulation network in chloroplasts. In this study, we developed a FRET-based sensor protein by combining CFP, YFP, and the N-terminal region of CP12, a redox-sensitive regulatory and Trx-targeted protein in chloroplasts. This sensor protein enabled us to monitor the redox change of chloroplast thioredoxin <i>in vivo</i>, and we therefore designated this protein \"chan"],"journal":["The Journal of biological chemistry"],"pubmed_title":["The thioredoxin (Trx) redox state sensor protein can visualize Trx activities in the light/dark response in chloroplasts."],"pmcid":["PMC6690705"],"funding_grant_id":["16H06556"],"pubmed_authors":["Sugiura K","Yokochi Y","Fukaya Y","Mihara S","Fu N","Yoshida K","Hisabori T"],"additional_accession":[]},"is_claimable":false,"name":"The thioredoxin (Trx) redox state sensor protein can visualize Trx activities in the light/dark response in chloroplasts.","description":"Thiol-based redox regulation via ferredoxin-thioredoxin (Trx) reductase/Trx controls various functions in chloroplasts in response to light/dark changes. Trx is a key factor of this regulatory system, and five Trx subtypes, including 10 isoforms, have been identified as chloroplast-localized forms in <i>Arabidopsis thaliana</i> These subtypes display distinct target selectivity, and, consequently, they form a complicated redox regulation network in chloroplasts. In this study, we developed a FRET-based sensor protein by combining CFP, YFP, and the N-terminal region of CP12, a redox-sensitive regulatory and Trx-targeted protein in chloroplasts. This sensor protein enabled us to monitor the redox change of chloroplast thioredoxin <i>in vivo</i>, and we therefore designated this protein \"chan","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Aug","modification":"2025-04-05T15:17:44.428Z","creation":"2025-04-05T15:17:44.428Z"},"accession":"S-EPMC6690705","cross_references":{"pubmed":["31217277"],"doi":["10.1074/jbc.RA119.007616","10.1074/jbc.ra119.007616"]}}