{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song P"],"funding":["China Scholarship Council","Center for Individualized Management of Tissue Damage and Regeneration","Danish National Research Foundation for Center for Cellular Signal Patterns","The Danish Cancer Society","Novo Nordisk Fonden","Lundbeck Foundation","VELUX Foundation"],"pagination":["1424-1435"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6697342"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(8)"],"pubmed_abstract":["Interleukin-1 beta (IL-1β) plays a central role in the induction of rheumatoid arthritis (RA). In the present study, we demonstrated that lipidoid-polymer hybrid nanoparticle (FS14-NP) can efficiently deliver siRNA against IL-1β (siIL-1β) to macrophages and effectively suppress the pathogenesis of experimental arthritis induced by collagen antibody (CAIA mice). FS14-NP/siIL-1β achieved approximately 70% and 90% gene-silencing efficiency in the RAW 264.7 cell line and intraperitoneal macrophages, respectively. Intravenous administration of FS14-NP/siRNA led to rapid accumulation of siRNA in macrophages within the arthritic joints. Furthermore, FS14-NP/siIL-1β treatment lowered the expression of pro-inflammatory cytokines in arthritic joints and dramatically attenuated ankle swelling, bone erosion, and cartilage destruction. These results demonstrate that FS14-NP/siIL-1β may represent an effective therapy for systemic arthritis and other inflammatory disorders."],"journal":["Molecular therapy : the journal of the American Society of Gene Therapy"],"pubmed_title":["Lipidoid-siRNA Nanoparticle-Mediated IL-1β Gene Silencing for Systemic Arthritis Therapy in a Mouse Model."],"pmcid":["PMC6697342"],"funding_grant_id":["NNF14OC0013407","Project No. 23750","R146-A9588","NNF17OC0028070","NNF16OC0020586"],"pubmed_authors":["Thomsen JS","Kjems J","Song P","Deleuran B","Jakobsen M","Yang C","Bruel A","Dagnæs-Hansen F"],"additional_accession":[]},"is_claimable":false,"name":"Lipidoid-siRNA Nanoparticle-Mediated IL-1β Gene Silencing for Systemic Arthritis Therapy in a Mouse Model.","description":"Interleukin-1 beta (IL-1β) plays a central role in the induction of rheumatoid arthritis (RA). In the present study, we demonstrated that lipidoid-polymer hybrid nanoparticle (FS14-NP) can efficiently deliver siRNA against IL-1β (siIL-1β) to macrophages and effectively suppress the pathogenesis of experimental arthritis induced by collagen antibody (CAIA mice). FS14-NP/siIL-1β achieved approximately 70% and 90% gene-silencing efficiency in the RAW 264.7 cell line and intraperitoneal macrophages, respectively. Intravenous administration of FS14-NP/siRNA led to rapid accumulation of siRNA in macrophages within the arthritic joints. Furthermore, FS14-NP/siIL-1β treatment lowered the expression of pro-inflammatory cytokines in arthritic joints and dramatically attenuated ankle swelling, bone erosion, and cartilage destruction. These results demonstrate that FS14-NP/siIL-1β may represent an effective therapy for systemic arthritis and other inflammatory disorders.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Aug","modification":"2025-04-22T10:39:07.928Z","creation":"2025-04-05T23:38:24.662Z"},"accession":"S-EPMC6697342","cross_references":{"pubmed":["31153827"],"doi":["10.1016/j.ymthe.2019.05.002"]}}