{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tornabene E"],"funding":["Lundbeckfonden","Novo Nordisk Fonden","Lundbeck Foundation"],"pagination":["e0221103"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6699694"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(8)"],"pubmed_abstract":["Ischemic stroke has been shown to induce breakdown of the blood-brain barrier, although these changes are not fully characterized. Oxygen-glucose deprivation (OGD) has been used to investigate the effects of ischemia in cultured brain capillary endothelial cells, however this involves a change of medium which in itself may affect the cells. The aim of the present study was to investigate the effect of OGD and simple medium exchange followed by 48 h of reperfusion on barrier properties of primary bovine endothelial cells co-cultured with rat astrocytes. Barrier properties were evaluated by transendothelial electrical resistance measurements, passive permeability of flux markers, RT-qPCR and immunocytochemistry. Both OGD and simple medium exchange caused an increase in endothelial monolayer "],"journal":["PloS one"],"pubmed_title":["Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures."],"pmcid":["PMC6699694"],"funding_grant_id":["NNF16OC0023194","R302-2018-2266"],"pubmed_authors":["Helms HCC","Pedersen SF","Brodin B","Tornabene E"],"additional_accession":[]},"is_claimable":false,"name":"Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures.","description":"Ischemic stroke has been shown to induce breakdown of the blood-brain barrier, although these changes are not fully characterized. Oxygen-glucose deprivation (OGD) has been used to investigate the effects of ischemia in cultured brain capillary endothelial cells, however this involves a change of medium which in itself may affect the cells. The aim of the present study was to investigate the effect of OGD and simple medium exchange followed by 48 h of reperfusion on barrier properties of primary bovine endothelial cells co-cultured with rat astrocytes. Barrier properties were evaluated by transendothelial electrical resistance measurements, passive permeability of flux markers, RT-qPCR and immunocytochemistry. Both OGD and simple medium exchange caused an increase in endothelial monolayer ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-20T03:27:11.394Z","creation":"2025-04-20T03:27:11.394Z"},"accession":"S-EPMC6699694","cross_references":{"pubmed":["31425564"],"doi":["10.1371/journal.pone.0221103"]}}