biostudies-literatureLiu JNational Natural Science Foundation of ChinaNational Key Research and Development Program of China262https://www.ebi.ac.uk/biostudies/studies/S-EPMC6708172biostudies-literatureUnknown10(1)<h4>Background</h4>The efficacy of mesenchymal stem cell (MSC)-based therapy for acute liver injury (ALI) involves coordination with the hepatic immune system, a complex and coordinated network of immune-cell interactions. However, studies of the immunomodulatory effects of MSCs have focused on a limited number of cell subsets rather than a systematic assessment.<h4>Methods</h4>Carbon tetrachloride (CCl₄) was used to induce ALI in mice. To determine the efficacy of MSCs, ALI mice were injected with MSCs via the tail vein, and histopathological changes, survival rate, and the serum levels of liver enzymes were determined. To assess the immune response induced by MSCs, a mass cytometry panel of 43 metal isotope-tagged antibodies was used to characterize the hepatic immune compartmStem cell research & therapyImmunomodulatory effect of mesenchymal stem cells in chemical-induced liver injury: a high-dimensional analysis.PMC67081722016YFA010100181620108028Sheng XLiu JCao HFeng XZeng XFeng BYu JLi LShi XZhu JChen WPan QXu YfalseImmunomodulatory effect of mesenchymal stem cells in chemical-induced liver injury: a high-dimensional analysis.<h4>Background</h4>The efficacy of mesenchymal stem cell (MSC)-based therapy for acute liver injury (ALI) involves coordination with the hepatic immune system, a complex and coordinated network of immune-cell interactions. However, studies of the immunomodulatory effects of MSCs have focused on a limited number of cell subsets rather than a systematic assessment.<h4>Methods</h4>Carbon tetrachloride (CCl₄) was used to induce ALI in mice. To determine the efficacy of MSCs, ALI mice were injected with MSCs via the tail vein, and histopathological changes, survival rate, and the serum levels of liver enzymes were determined. To assess the immune response induced by MSCs, a mass cytometry panel of 43 metal isotope-tagged antibodies was used to characterize the hepatic immune compartm2019-01-01T00:00:00Z2019 Aug2025-04-26T02:16:32.1Z2019-09-05T07:01:50ZS-EPMC67081723144368610.1186/s13287-019-1379-6