biostudies-literatureUnknown14(8)Zeh NExosomes represent a promising delivery tool for nucleic acid-based pharmaceuticals. They are highly suitable for transporting therapeutic miRNAs to tumor cells, due to their natural membrane components. Further, exosomes are capable of effectively protecting nucleic acids against ribonucleases and enable the delivery of their content through cell membranes. However, no suitable production host for miRNA containing exosomes of non-tumorigenic origin has yet been identified. In this study we engineered an immortalised human amniocyte cell line (CAP® cells), whose exosomes were enriched and characterised. The cell line modifications not only enabled the production of GFP-labelled but also pro-apoptotic miRNA containing exosomes without negative influence on host cell growth. Furthermore, we PloS onee0221679https://www.ebi.ac.uk/biostudies/studies/S-EPMC6713437biostudies-literatureHuman CAP cells represent a novel source for functional, miRNA-loaded exosome production.PMC6713437Schneider HMathias SWissing SHandrick ROtte KZeh NKleemann MStrempel NSchmidt-Hertel SWeis BRaab NfalseHuman CAP cells represent a novel source for functional, miRNA-loaded exosome production.Exosomes represent a promising delivery tool for nucleic acid-based pharmaceuticals. They are highly suitable for transporting therapeutic miRNAs to tumor cells, due to their natural membrane components. Further, exosomes are capable of effectively protecting nucleic acids against ribonucleases and enable the delivery of their content through cell membranes. However, no suitable production host for miRNA containing exosomes of non-tumorigenic origin has yet been identified. In this study we engineered an immortalised human amniocyte cell line (CAP® cells), whose exosomes were enriched and characterised. The cell line modifications not only enabled the production of GFP-labelled but also pro-apoptotic miRNA containing exosomes without negative influence on host cell growth. Furthermore, we 2019-01-01T00:00:00Z20192025-04-19T11:55:30.074Z2019-09-08T07:01:15ZS-EPMC67134373146148610.1371/journal.pone.0221679