{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Toni LS"],"funding":["NHLBI NIH HHS"],"pagination":["e0221519"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6716635"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(8)"],"pubmed_abstract":["<h4>Objectives</h4>To investigate the biologic relevance of cross-platform concordant changes in gene expression in intact human failing/hypertrophied ventricular myocardium undergoing reverse remodeling.<h4>Background</h4>Information is lacking on genes and networks involved in remodeled human LVs, and in the associated investigative best practices.<h4>Methods</h4>We measured mRNA expression in ventricular septal endomyocardial biopsies from 47 idiopathic dilated cardiomyopathy patients, at baseline and after 3-12 months of β-blocker treatment to effect left ventricular (LV) reverse remodeling as measured by ejection fraction (LVEF). Cross-platform gene expression change concordance was investigated in reverse remodeling Responders (R) and Nonresponders (NR) using 3 platforms (RT-qPCR, mi"],"journal":["PloS one"],"pubmed_title":["Sequential analysis of myocardial gene expression with phenotypic change: Use of cross-platform concordance to strengthen biologic relevance."],"pmcid":["PMC6716635"],"funding_grant_id":["R01 HL071118","T32 HL007822"],"pubmed_authors":["Minobe WA","Lowes BD","Toni LS","Schwisow JA","Rodriguez EM","Buttrick PM","Altman NL","Kao DP","Bristow MR","Carroll IA","Jones KL","Gilbert EM"],"additional_accession":[]},"is_claimable":false,"name":"Sequential analysis of myocardial gene expression with phenotypic change: Use of cross-platform concordance to strengthen biologic relevance.","description":"<h4>Objectives</h4>To investigate the biologic relevance of cross-platform concordant changes in gene expression in intact human failing/hypertrophied ventricular myocardium undergoing reverse remodeling.<h4>Background</h4>Information is lacking on genes and networks involved in remodeled human LVs, and in the associated investigative best practices.<h4>Methods</h4>We measured mRNA expression in ventricular septal endomyocardial biopsies from 47 idiopathic dilated cardiomyopathy patients, at baseline and after 3-12 months of β-blocker treatment to effect left ventricular (LV) reverse remodeling as measured by ejection fraction (LVEF). Cross-platform gene expression change concordance was investigated in reverse remodeling Responders (R) and Nonresponders (NR) using 3 platforms (RT-qPCR, mi","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-19T16:44:31.42Z","creation":"2019-09-24T07:05:47Z"},"accession":"S-EPMC6716635","cross_references":{"pubmed":["31469842"],"doi":["10.1371/journal.pone.0221519"]}}