biostudies-literatureToni LSNHLBI NIH HHSe0221519https://www.ebi.ac.uk/biostudies/studies/S-EPMC6716635biostudies-literatureUnknown14(8)<h4>Objectives</h4>To investigate the biologic relevance of cross-platform concordant changes in gene expression in intact human failing/hypertrophied ventricular myocardium undergoing reverse remodeling.<h4>Background</h4>Information is lacking on genes and networks involved in remodeled human LVs, and in the associated investigative best practices.<h4>Methods</h4>We measured mRNA expression in ventricular septal endomyocardial biopsies from 47 idiopathic dilated cardiomyopathy patients, at baseline and after 3-12 months of β-blocker treatment to effect left ventricular (LV) reverse remodeling as measured by ejection fraction (LVEF). Cross-platform gene expression change concordance was investigated in reverse remodeling Responders (R) and Nonresponders (NR) using 3 platforms (RT-qPCR, miPloS oneSequential analysis of myocardial gene expression with phenotypic change: Use of cross-platform concordance to strengthen biologic relevance.PMC6716635R01 HL071118T32 HL007822Minobe WALowes BDToni LSSchwisow JARodriguez EMButtrick PMAltman NLKao DPBristow MRCarroll IAJones KLGilbert EMfalseSequential analysis of myocardial gene expression with phenotypic change: Use of cross-platform concordance to strengthen biologic relevance.<h4>Objectives</h4>To investigate the biologic relevance of cross-platform concordant changes in gene expression in intact human failing/hypertrophied ventricular myocardium undergoing reverse remodeling.<h4>Background</h4>Information is lacking on genes and networks involved in remodeled human LVs, and in the associated investigative best practices.<h4>Methods</h4>We measured mRNA expression in ventricular septal endomyocardial biopsies from 47 idiopathic dilated cardiomyopathy patients, at baseline and after 3-12 months of β-blocker treatment to effect left ventricular (LV) reverse remodeling as measured by ejection fraction (LVEF). Cross-platform gene expression change concordance was investigated in reverse remodeling Responders (R) and Nonresponders (NR) using 3 platforms (RT-qPCR, mi2019-01-01T00:00:00Z20192025-04-19T16:44:31.42Z2019-09-24T07:05:47ZS-EPMC67166353146984210.1371/journal.pone.0221519