biostudies-literatureMubaid SNHGRI NIH HHSProstate Cancer CanadaGouvernement du Canada | Canadian Institutes of Health ResearchCIHR17261-17270https://www.ebi.ac.uk/biostudies/studies/S-EPMC6717253biostudies-literatureUnknown116(35)Debilitating cancer-induced muscle wasting, a syndrome known as cachexia, is lethal. Here we report a posttranscriptional pathway involving the RNA-binding protein HuR as a key player in the onset of this syndrome. Under these conditions, HuR switches its function from a promoter of muscle fiber formation to become an inducer of muscle loss. HuR binds to the <i>STAT3</i> (<i>signal transducer and activator of transcription 3</i>) mRNA, which encodes one of the main effectors of this condition, promoting its expression both in vitro and in vivo. While HuR does not affect the stability and the cellular movement of this transcript, HuR promotes the translation of the <i>STAT3</i> mRNA by preventing miR-330 (microRNA 330)-mediated translation inhibition. To achieve this effect, HuR directly biProceedings of the National Academy of Sciences of the United States of AmericaHuR counteracts miR-330 to promote STAT3 translation during inflammation-induced muscle wasting.PMC6717253D2014-14R21 HG003679FRN53888U01 HG004571MOP-142399Ma JFMubaid SAshour KLian XJCammas ASanchez BJChittur SVRobinson SGallouzi IETenenbaum SAOmer ADi Marco SDormoy-Raclet VfalseHuR counteracts miR-330 to promote STAT3 translation during inflammation-induced muscle wasting.Debilitating cancer-induced muscle wasting, a syndrome known as cachexia, is lethal. Here we report a posttranscriptional pathway involving the RNA-binding protein HuR as a key player in the onset of this syndrome. Under these conditions, HuR switches its function from a promoter of muscle fiber formation to become an inducer of muscle loss. HuR binds to the <i>STAT3</i> (<i>signal transducer and activator of transcription 3</i>) mRNA, which encodes one of the main effectors of this condition, promoting its expression both in vitro and in vivo. While HuR does not affect the stability and the cellular movement of this transcript, HuR promotes the translation of the <i>STAT3</i> mRNA by preventing miR-330 (microRNA 330)-mediated translation inhibition. To achieve this effect, HuR directly bi2019-01-01T00:00:00Z2019 Aug2025-04-04T20:10:24.378Z2025-04-04T20:10:24.378ZS-EPMC67172533140598910.1073/pnas.1905172116