biostudies-literatureChen Xthe Program for Changjiang Scholars and Innovative Research Team in Universitythe Scientific Research Innovation Project in Shihezi Universitythe Xinjiang Science and Technology Major ProjectE2950https://www.ebi.ac.uk/biostudies/studies/S-EPMC6720801biostudies-literatureUnknown24(16)Utilizing a pharmacophore hybridization approach, we have designed and synthesized a novel series of 28 new heterobivalent β-carbolines. The in vitro cytotoxic potential of each compound was evaluated against the five cancer cell lines (LLC, BGC-823, CT-26, Bel-7402, and MCF-7) of different origin-murine and human, with the aim of determining the potency and selectivity of the compounds. Compound <b>8z</b> showed antitumor activities with half-maximal inhibitory concentration (IC₅₀) values of 9.9 ± 0.9, 8.6 ± 1.4, 6.2 ± 2.5, 9.9 ± 0.5, and 5.7 ± 1.2 µM against the tested five cancer cell lines. Moreover, the effect of compound <b>8z</b> on the angiogenesis process was investigated using a chicken chorioallantoic membrane (CAM) in vivo model. At a concentration of 5 μM, compound Molecules (Basel, Switzerland)Design, Synthesis, and Biological Evaluation of Novel N-Acylhydrazone Bond Linked Heterobivalent β-Carbolines as Potential Anticancer Agents.PMC6720801IRT15R462016A03005-1SHYL-YB201804Ma QGuo LFan WZhang JChen WChen XfalseDesign, Synthesis, and Biological Evaluation of Novel N-Acylhydrazone Bond Linked Heterobivalent β-Carbolines as Potential Anticancer Agents.Utilizing a pharmacophore hybridization approach, we have designed and synthesized a novel series of 28 new heterobivalent β-carbolines. The in vitro cytotoxic potential of each compound was evaluated against the five cancer cell lines (LLC, BGC-823, CT-26, Bel-7402, and MCF-7) of different origin-murine and human, with the aim of determining the potency and selectivity of the compounds. Compound <b>8z</b> showed antitumor activities with half-maximal inhibitory concentration (IC₅₀) values of 9.9 ± 0.9, 8.6 ± 1.4, 6.2 ± 2.5, 9.9 ± 0.5, and 5.7 ± 1.2 µM against the tested five cancer cell lines. Moreover, the effect of compound <b>8z</b> on the angiogenesis process was investigated using a chicken chorioallantoic membrane (CAM) in vivo model. At a concentration of 5 μM, compound 2019-01-01T00:00:00Z2019 Aug2026-05-06T21:32:39.752Z2019-09-14T07:04:45ZS-EPMC67208013141627110.3390/molecules24162950