{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Puig N"],"funding":["Generalitat de Catalunya","Instituto de Salud Carlos III"],"pagination":["E300"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6722802"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(8)"],"pubmed_abstract":["Electronegative low-density lipoprotein (LDL(-)) is a minor LDL subfraction that is present in blood with inflammatory and apoptotic effects. We aimed to evaluate the role of sphingolipids ceramide (Cer), sphingosine (Sph), and sphingosine-1-phosphate (S1P) in the LDL(-)-induced effect on monocytes. Total LDL was subfractioned into native LDL and LDL(-) by anion-exchange chromatography and their sphingolipid content evaluated by mass spectrometry. LDL subfractions were incubated with monocytes in the presence or absence of enzyme inhibitors: chlorpromazine (CPZ), d-erythro-2-(N-myristoyl amino)-1-phenyl-1-propanol (MAPP), and N,N-dimethylsphingosine (DMS), which inhibit Cer, Sph, and S1P generation, respectively. After incubation, we evaluated cytokine release by enzyme-linked immunosorben"],"journal":["Biomolecules"],"pubmed_title":["The Role of Distinctive Sphingolipids in the Inflammatory and Apoptotic Effects of Electronegative LDL on Monocytes."],"pmcid":["PMC6722802"],"funding_grant_id":["2017-SGR-1149","CIBERDEM CB07/08/0016","FIS PI13/00364 and FIS PI16/00471"],"pubmed_authors":["Puig N","Estruch M","Benitez S","Sanchez-Quesada JL","Jin L"],"additional_accession":[]},"is_claimable":false,"name":"The Role of Distinctive Sphingolipids in the Inflammatory and Apoptotic Effects of Electronegative LDL on Monocytes.","description":"Electronegative low-density lipoprotein (LDL(-)) is a minor LDL subfraction that is present in blood with inflammatory and apoptotic effects. We aimed to evaluate the role of sphingolipids ceramide (Cer), sphingosine (Sph), and sphingosine-1-phosphate (S1P) in the LDL(-)-induced effect on monocytes. Total LDL was subfractioned into native LDL and LDL(-) by anion-exchange chromatography and their sphingolipid content evaluated by mass spectrometry. LDL subfractions were incubated with monocytes in the presence or absence of enzyme inhibitors: chlorpromazine (CPZ), d-erythro-2-(N-myristoyl amino)-1-phenyl-1-propanol (MAPP), and N,N-dimethylsphingosine (DMS), which inhibit Cer, Sph, and S1P generation, respectively. After incubation, we evaluated cytokine release by enzyme-linked immunosorben","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jul","modification":"2025-04-04T19:02:05.041Z","creation":"2019-09-14T07:05:32Z"},"accession":"S-EPMC6722802","cross_references":{"pubmed":["31344975"],"doi":["10.3390/biom9080300"]}}