biostudies-literatureChuanboon KYoung Research ScholarMahidol UniversityNational Research Council of Thailande6904https://www.ebi.ac.uk/biostudies/studies/S-EPMC6727835biostudies-literatureUnknown7<h4>Background</h4><i>Enterobacter cloacae</i> (EC) is a Gram-negative bacterium that has been utilized extensively in biotechnological and environmental science applications, possibly because of its high capability for adapting itself and surviving in hazardous conditions. A search for the EC from agricultural and industrial areas that possesses high capability to tolerate and/or accumulate cadmium ions has been conducted in this study. Plausible mechanisms of cellular adaptations in the presence of toxic cadmium have also been proposed.<h4>Methods</h4>Nine strains of EC were isolated and subsequently identified by biochemical characterization and MALDI-Biotyper. Minimum inhibitory concentrations (MICs) against cadmium, zinc and copper ions were determined by agar dilution method. Growth PeerJProteomics and bioinformatics analysis reveal potential roles of cadmium-binding proteins in cadmium tolerance and accumulation of <i>Enterobacter cloacae</i>.PMC6727835MRG5480205B.E. 2557-2559B.E. 2561-2562Na Nakorn PIsarankura-Na-Ayudhya CChuanboon KPannengpetch SIsarankura-Na-Ayudhya PNuchnoi PLaengsri VfalseProteomics and bioinformatics analysis reveal potential roles of cadmium-binding proteins in cadmium tolerance and accumulation of <i>Enterobacter cloacae</i>.<h4>Background</h4><i>Enterobacter cloacae</i> (EC) is a Gram-negative bacterium that has been utilized extensively in biotechnological and environmental science applications, possibly because of its high capability for adapting itself and surviving in hazardous conditions. A search for the EC from agricultural and industrial areas that possesses high capability to tolerate and/or accumulate cadmium ions has been conducted in this study. Plausible mechanisms of cellular adaptations in the presence of toxic cadmium have also been proposed.<h4>Methods</h4>Nine strains of EC were isolated and subsequently identified by biochemical characterization and MALDI-Biotyper. Minimum inhibitory concentrations (MICs) against cadmium, zinc and copper ions were determined by agar dilution method. Growth 2019-01-01T00:00:00Z20192026-05-02T03:35:58.448Z2019-09-26T07:03:59ZS-EPMC67278353153483310.7717/peerj.6904