biostudies-literatureNajahi-Missaoui WU.S. Department of DefenseNational Institutes of HealthNational Institute of Biomedical Imaging and Bioengineeringe00518https://www.ebi.ac.uk/biostudies/studies/S-EPMC6728842biostudies-literatureUnknown7(5)P21-activated kinase-1 (PAK-1) is a serine/threonine kinase involved in multiple signaling pathways that mediate cellular functions such as cytoskeletal motility, cell proliferation, and survival. PAK-1 expression is altered in various cancers, including prostate and breast. Our recent studies showed that prostate cancer cells expressing higher levels of PAK-1 were resistant to the cytotoxic effects of the PAK-1 inhibitor, inhibitor targeting PAK-1 activation-3 (IPA-3), compared to those with lower expression. This study expanded these findings to other cancers (breast and melanoma) by testing the hypothesis that genetic and pharmacological inhibition of PAK-1 alters cell growth, migration, and invasion in prostate, breast, and skin cancer cell lines. We also tested the specificity of IPA-Pharmacology research & perspectivesEffect of P21-activated kinase 1 (PAK-1) inhibition on cancer cell growth, migration, and invasion.PMC672884211996600EB0160100Somanath PRQuach NDJenkins ADabke ICummings BSNajahi-Missaoui WfalseEffect of P21-activated kinase 1 (PAK-1) inhibition on cancer cell growth, migration, and invasion.P21-activated kinase-1 (PAK-1) is a serine/threonine kinase involved in multiple signaling pathways that mediate cellular functions such as cytoskeletal motility, cell proliferation, and survival. PAK-1 expression is altered in various cancers, including prostate and breast. Our recent studies showed that prostate cancer cells expressing higher levels of PAK-1 were resistant to the cytotoxic effects of the PAK-1 inhibitor, inhibitor targeting PAK-1 activation-3 (IPA-3), compared to those with lower expression. This study expanded these findings to other cancers (breast and melanoma) by testing the hypothesis that genetic and pharmacological inhibition of PAK-1 alters cell growth, migration, and invasion in prostate, breast, and skin cancer cell lines. We also tested the specificity of IPA-2019-01-01T00:00:00Z2019 Oct2025-04-04T21:30:32.554Z2019-09-24T07:08:06ZS-EPMC67288423151671310.1002/prp2.518