biostudies-literatureNooh MMThe University of Tennessee-HSCHHS | National Institutes of Health (NIH)NHLBI NIH HHSF375-F387https://www.ebi.ac.uk/biostudies/studies/S-EPMC6732448biostudies-literatureUnknown317(2)Arginine-vasopressin (AVP)-mediated translocation of aquaporin-2 (AQP2) protein-forming water channels from storage vesicles to the membrane of renal collecting ducts is critical for the renal conservation of water. The type-1 PDZ-binding motif (PBM) in AQP2, "GTKA," is a critical barcode for its translocation, but its precise role and that of its interacting protein partners in this process remain obscure. We determined that synapse-associated protein-97 (SAP97), a membrane-associated guanylate kinase protein involved in establishing epithelial cell polarity, was an avid binding partner to the PBM of AQP2. The role of PBM and SAP97 on AQP2 redistribution in response to AVP was assessed in LLC-PK₁ renal collecting cells by confocal microscopy and cell surface biotinylation technAmerican journal of physiology. Renal physiologyInvolvement of PDZ-SAP97 interactions in regulating AQP2 translocation in response to vasopressin in LLC-PK₁ cells.PMC6732448Bridge-funding 2016R01 HL085848HL-085848Kale ABahouth SWNooh MMfalseInvolvement of PDZ-SAP97 interactions in regulating AQP2 translocation in response to vasopressin in LLC-PK₁ cells.Arginine-vasopressin (AVP)-mediated translocation of aquaporin-2 (AQP2) protein-forming water channels from storage vesicles to the membrane of renal collecting ducts is critical for the renal conservation of water. The type-1 PDZ-binding motif (PBM) in AQP2, "GTKA," is a critical barcode for its translocation, but its precise role and that of its interacting protein partners in this process remain obscure. We determined that synapse-associated protein-97 (SAP97), a membrane-associated guanylate kinase protein involved in establishing epithelial cell polarity, was an avid binding partner to the PBM of AQP2. The role of PBM and SAP97 on AQP2 redistribution in response to AVP was assessed in LLC-PK₁ renal collecting cells by confocal microscopy and cell surface biotinylation techn2019-01-01T00:00:00Z2019 Aug2025-04-18T14:50:16.127Z2020-11-19T13:34:32ZS-EPMC67324483114139510.1152/ajprenal.00228.2018