biostudies-literatureUnknown9(20)Chen Z<b>Background & Aims</b>: The use of antisense oligonucleotide-based nanosystems for the detection and regulation of tumor-related gene expression is thought to be a promising approach for cancer diagnostics and therapies. Herein, we report that a cubic-shaped iron oxide nanoparticle (IONC) core nanobeacon is capable of delivering an HSP90α mRNA-specific molecular beacon (HSP90-MB) into living cells and enhancing <i>T</i> ₂-weighted MR imaging in a tumor model. <b>Methods</b>: The nanobeacons were built with IONC, generation 4 poly(amidoamine) dendrimer (G4 PAMAM), Pluronic P123 (P123) and HSP90-MB labeled with a quencher (BHQ1) and a fluorophore (Alexa Fluor 488). <b>Results</b>: After internalization by malignant cells overexpressing HSP90α, the fluorescence of the nanobeacon Theranostics5784-5796https://www.ebi.ac.uk/biostudies/studies/S-EPMC6735378biostudies-literatureDendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging.PMC6735378Yang HPeng YXie XFeng YQin XWu CLiu YLi SZhu JChen ZLi TZheng CYou FfalseDendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging.<b>Background & Aims</b>: The use of antisense oligonucleotide-based nanosystems for the detection and regulation of tumor-related gene expression is thought to be a promising approach for cancer diagnostics and therapies. Herein, we report that a cubic-shaped iron oxide nanoparticle (IONC) core nanobeacon is capable of delivering an HSP90α mRNA-specific molecular beacon (HSP90-MB) into living cells and enhancing <i>T</i> ₂-weighted MR imaging in a tumor model. <b>Methods</b>: The nanobeacons were built with IONC, generation 4 poly(amidoamine) dendrimer (G4 PAMAM), Pluronic P123 (P123) and HSP90-MB labeled with a quencher (BHQ1) and a fluorophore (Alexa Fluor 488). <b>Results</b>: After internalization by malignant cells overexpressing HSP90α, the fluorescence of the nanobeacon 2019-01-01T00:00:00Z20192025-04-04T08:30:14.322Z2019-09-26T07:05:06ZS-EPMC67353783153451910.7150/thno.36545