biostudies-literatureUnknown18(1)Wang L<h4>Background</h4>Mitochondrial and cytoplasmic malate transporter proteins are responsible for transmembrane transport of malate, thereby linking malate metabolism in various subcellular regions of the cell. These transporters play an important role in fatty acid biosynthesis of oleaginous microorganisms. Our previous studies have found that lipid content of the recombinant Mucor circinelloides (M. circinelloides) strain with mitochondrial malate transporter (mt) gene overexpression was increased by 70%, while that of strain with mt gene knockout was decreased by 27%. However, the mechanism of malate transporter promoting the transport of mitochondrial malate and citrate related to lipid accumulation is not clear. Therefore, ¹³C-labeled glucose metabolic flux analysis was carrMicrobial cell factories154https://www.ebi.ac.uk/biostudies/studies/S-EPMC6737672biostudies-literature¹³C metabolic flux analysis on roles of malate transporter in lipid accumulation of Mucor circinelloides.PMC6737672Zhang YZhang HWang LSong Yfalse¹³C metabolic flux analysis on roles of malate transporter in lipid accumulation of Mucor circinelloides.<h4>Background</h4>Mitochondrial and cytoplasmic malate transporter proteins are responsible for transmembrane transport of malate, thereby linking malate metabolism in various subcellular regions of the cell. These transporters play an important role in fatty acid biosynthesis of oleaginous microorganisms. Our previous studies have found that lipid content of the recombinant Mucor circinelloides (M. circinelloides) strain with mitochondrial malate transporter (mt) gene overexpression was increased by 70%, while that of strain with mt gene knockout was decreased by 27%. However, the mechanism of malate transporter promoting the transport of mitochondrial malate and citrate related to lipid accumulation is not clear. Therefore, ¹³C-labeled glucose metabolic flux analysis was carr2019-01-01T00:00:00Z2019 Sep2025-04-19T09:39:01.172Z2019-09-24T07:10:06ZS-EPMC67376723150610110.1186/s12934-019-1207-9