biostudies-literatureCasares LCancer Research UKEuropean UnionMinistry of the Economy and CompetitionMedical Research Institute of the University of DundeeFEDERTenovus Scotland101321https://www.ebi.ac.uk/biostudies/studies/S-EPMC6742916biostudies-literatureUnknown28Cannabidiol (CBD) is a major non-psychotropic phytocannabinoid that attracted a great attention for its therapeutic potential against different pathologies including skin diseases. However, although the efficacy in preclinical models and the clinical benefits of CBD in humans have been extensively demonstrated, the molecular mechanism(s) and targets responsible for these effects are as yet unknown. Herein we characterized at the molecular level the effects of CBD on primary human keratinocytes using a combination of RNA sequencing (RNA-Seq) and sequential window acquisition of all theoretical mass spectrometry (SWATH-MS). Functional analysis revealed that CBD regulated pathways involved in keratinocyte differentiation, skin development and epidermal cell differentiation among other processRedox biologyCannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1.PMC6742916C52419/A2286922869T18/07A22869SAF2017-87701-RC52419Garrido-Rodriguez MCasares Lde la Vega LGarcia VPenarando JCollado JACalzado MAMillan EGarcia-Martin AMunoz EfalseCannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1.Cannabidiol (CBD) is a major non-psychotropic phytocannabinoid that attracted a great attention for its therapeutic potential against different pathologies including skin diseases. However, although the efficacy in preclinical models and the clinical benefits of CBD in humans have been extensively demonstrated, the molecular mechanism(s) and targets responsible for these effects are as yet unknown. Herein we characterized at the molecular level the effects of CBD on primary human keratinocytes using a combination of RNA sequencing (RNA-Seq) and sequential window acquisition of all theoretical mass spectrometry (SWATH-MS). Functional analysis revealed that CBD regulated pathways involved in keratinocyte differentiation, skin development and epidermal cell differentiation among other process2020-01-01T00:00:00Z2020 Jan2026-05-01T15:04:06.243Z2026-04-07T16:58:29.386ZS-EPMC67429163151889210.1016/j.redox.2019.101321