{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu H"],"funding":["Intramural NIH HHS","NIDDK NIH HHS"],"pagination":["2298-306"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6754265"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["58(10)"],"pubmed_abstract":["<h4>Aims/hypothesis</h4>miR-153 is an intronic miRNA embedded in the genes that encode IA-2 (also known as PTPRN) and IA-2β (also known as PTPRN2). Islet antigen (IA)-2 and IA-2β are major autoantigens in type 1 diabetes and are important transmembrane proteins in dense core and synaptic vesicles. miR-153 and its host genes are co-regulated in pancreas and brain. The present experiments were initiated to decipher the regulatory network between miR-153 and its host gene Ia-2β (also known as Ptprn2).<h4>Methods</h4>Insulin secretion was determined by ELISA. Identification of miRNA targets was assessed using luciferase assays and by quantitative real-time PCR and western blots in vitro and in vivo. Target protector was also employed to evaluate miRNA target function.<h4>Results</h4>Functional"],"journal":["Diabetologia"],"pubmed_title":["The Ia-2β intronic miRNA, miR-153, is a negative regulator of insulin and dopamine secretion through its effect on the Cacna1c gene in mice."],"pmcid":["PMC6754265"],"funding_grant_id":["R01 DK046409","R01DK46409"],"pubmed_authors":["Vadrevu S","Carmona GN","Satin LS","Abuhatzira L","Notkins AL","Xu H"],"additional_accession":[]},"is_claimable":false,"name":"The Ia-2β intronic miRNA, miR-153, is a negative regulator of insulin and dopamine secretion through its effect on the Cacna1c gene in mice.","description":"<h4>Aims/hypothesis</h4>miR-153 is an intronic miRNA embedded in the genes that encode IA-2 (also known as PTPRN) and IA-2β (also known as PTPRN2). Islet antigen (IA)-2 and IA-2β are major autoantigens in type 1 diabetes and are important transmembrane proteins in dense core and synaptic vesicles. miR-153 and its host genes are co-regulated in pancreas and brain. The present experiments were initiated to decipher the regulatory network between miR-153 and its host gene Ia-2β (also known as Ptprn2).<h4>Methods</h4>Insulin secretion was determined by ELISA. Identification of miRNA targets was assessed using luciferase assays and by quantitative real-time PCR and western blots in vitro and in vivo. Target protector was also employed to evaluate miRNA target function.<h4>Results</h4>Functional","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Oct","modification":"2025-04-19T09:39:56.543Z","creation":"2019-10-03T07:05:19Z"},"accession":"S-EPMC6754265","cross_references":{"pubmed":["26141787"],"doi":["10.1007/s00125-015-3683-8"]}}