biostudies-literatureRavenhill BJEvelyn TrustNIAID NIH HHSNHLBI NIH HHSWellcome TrustCIHR350https://www.ebi.ac.uk/biostudies/studies/S-EPMC6754445biostudies-literatureUnknown2Red blood cells (RBCs) play a critical role in oxygen transport, and are the focus of important diseases including malaria and the haemoglobinopathies. Proteins at the RBC surface can determine susceptibility to disease, however previous studies classifying the RBC proteome have not used specific strategies directed at enriching cell surface proteins. Furthermore, there has been no systematic analysis of variation in abundance of RBC surface proteins between genetically disparate human populations. These questions are important to inform not only basic RBC biology but additionally to identify novel candidate receptors for malarial parasites. Here, we use 'plasma membrane profiling' and tandem mass tag-based mass spectrometry to enrich and quantify primary RBC cell surface proteins from twoCommunications biologyQuantitative comparative analysis of human erythrocyte surface proteins between individuals from two genetically distinct populations.PMC6754445108070/Z/15/Z100140R01 AI091787R01 HL139337Goldberg JMRavenhill BJDuraisingh MTKanjee UAhouidi AWeekes MPNobre LDieye TWilliamson JAntrobus RfalseQuantitative comparative analysis of human erythrocyte surface proteins between individuals from two genetically distinct populations.Red blood cells (RBCs) play a critical role in oxygen transport, and are the focus of important diseases including malaria and the haemoglobinopathies. Proteins at the RBC surface can determine susceptibility to disease, however previous studies classifying the RBC proteome have not used specific strategies directed at enriching cell surface proteins. Furthermore, there has been no systematic analysis of variation in abundance of RBC surface proteins between genetically disparate human populations. These questions are important to inform not only basic RBC biology but additionally to identify novel candidate receptors for malarial parasites. Here, we use 'plasma membrane profiling' and tandem mass tag-based mass spectrometry to enrich and quantify primary RBC cell surface proteins from two2019-01-01T00:00:00Z20192025-04-19T09:41:51.751Z2019-10-11T07:08:53ZS-EPMC67544453155230310.1038/s42003-019-0596-y