{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Aris M"],"funding":["Instituto Nacional del Cáncer"],"pubmed_abstract":["The CSF-470 cellular vaccine plus BCG and rhGM-CSF increased distant metastases-free survival in cutaneous melanoma patients stages IIB-IIC-III relative to medium dose IFN-α2b (CASVAC-0401 study). Patient-045 developed a mature vaccination site (VAC-SITE) and a regional cutaneous metastasis (C-MTS), which were excised during the protocol, remaining disease-free 36 months from vaccination start. CDR3-TCRβ repertoire sequencing in PBMC and tissue samples, along with skin-DTH score and IFN-γ ELISPOT assay, were performed to analyze the T-cell immune response dynamics throughout the immunization protocol. Histopathological analysis of the VAC-SITE revealed a highly-inflamed granulomatous structure encircled by CD11c+ nested-clusters, brisk CD8+ and scarce FOXP3+, lymphocytes with numerous Lang"],"journal":["Frontiers in immunology"],"pagination":["2213"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6759869"],"repository":["biostudies-literature"],"pubmed_title":["Immunization With the CSF-470 Vaccine Plus BCG and rhGM-CSF Induced in a Cutaneous Melanoma Patient a TCRβ Repertoire Found at Vaccination Site and Tumor Infiltrating Lymphocytes That Persisted in Blood."],"pmcid":["PMC6759869"],"pubmed_authors":["Bravo AI","Blanco PA","Bentivegna S","Carri I","Aris M","Garcia Alvarez HM","Barrio MM","Mordoh J","Nielsen M","Podaza E","Rotondaro C"],"additional_accession":[]},"is_claimable":false,"name":"Immunization With the CSF-470 Vaccine Plus BCG and rhGM-CSF Induced in a Cutaneous Melanoma Patient a TCRβ Repertoire Found at Vaccination Site and Tumor Infiltrating Lymphocytes That Persisted in Blood.","description":"The CSF-470 cellular vaccine plus BCG and rhGM-CSF increased distant metastases-free survival in cutaneous melanoma patients stages IIB-IIC-III relative to medium dose IFN-α2b (CASVAC-0401 study). Patient-045 developed a mature vaccination site (VAC-SITE) and a regional cutaneous metastasis (C-MTS), which were excised during the protocol, remaining disease-free 36 months from vaccination start. CDR3-TCRβ repertoire sequencing in PBMC and tissue samples, along with skin-DTH score and IFN-γ ELISPOT assay, were performed to analyze the T-cell immune response dynamics throughout the immunization protocol. Histopathological analysis of the VAC-SITE revealed a highly-inflamed granulomatous structure encircled by CD11c+ nested-clusters, brisk CD8+ and scarce FOXP3+, lymphocytes with numerous Lang","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-04T07:52:17.063Z","creation":"2019-10-30T08:17:30Z"},"accession":"S-EPMC6759869","cross_references":{"pubmed":["31620131"],"doi":["10.3389/fimmu.2019.02213"]}}