{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Blasius TL"],"funding":["NIDDK NIH HHS","National Institutes of Health","NIGMS NIH HHS","Japan Society for the Promotion of Science"],"pagination":["e0222924"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6760808"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(9)"],"pubmed_abstract":["Cilia are microtubule-based organelles that protrude from the surface of eukaryotic cells to generate motility and to sense and respond to environmental cues. In order to carry out these functions, the complement of proteins in the cilium must be specific for the organelle. Regulation of protein entry into primary cilia has been shown to utilize mechanisms and components of nuclear gating, including nucleoporins of the nuclear pore complex (NPC). We show that nucleoporins also localize to the base of motile cilia on the surface of trachea epithelial cells. How nucleoporins are anchored at the cilium base has been unclear as transmembrane nucleoporins, which anchor nucleoporins at the nuclear envelope, have not been found to localize at the cilium. Here we use the directed yeast two-hybrid "],"journal":["PloS one"],"pubmed_title":["NPHP proteins are binding partners of nucleoporins at the base of the primary cilium."],"pmcid":["PMC6760808"],"funding_grant_id":["R01 GM116204","P30 DK092926","R01GM116204","P30DK092926"],"pubmed_authors":["Blasius TL","Takao D","Verhey KJ"],"additional_accession":[]},"is_claimable":false,"name":"NPHP proteins are binding partners of nucleoporins at the base of the primary cilium.","description":"Cilia are microtubule-based organelles that protrude from the surface of eukaryotic cells to generate motility and to sense and respond to environmental cues. In order to carry out these functions, the complement of proteins in the cilium must be specific for the organelle. Regulation of protein entry into primary cilia has been shown to utilize mechanisms and components of nuclear gating, including nucleoporins of the nuclear pore complex (NPC). We show that nucleoporins also localize to the base of motile cilia on the surface of trachea epithelial cells. How nucleoporins are anchored at the cilium base has been unclear as transmembrane nucleoporins, which anchor nucleoporins at the nuclear envelope, have not been found to localize at the cilium. Here we use the directed yeast two-hybrid ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-04-07T15:40:28.139Z","creation":"2019-10-11T07:11:06Z"},"accession":"S-EPMC6760808","cross_references":{"pubmed":["31553752"],"doi":["10.1371/journal.pone.0222924"]}}