{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dias BT"],"funding":["Conselho Nacional de Desenvolvimento Científico e Tecnológico","Medical Research Council","Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro"],"pagination":["10794-10807"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6766642"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(10)"],"pubmed_abstract":["Visceral leishmaniasis is a deadly illness caused by <i>Leishmania donovani</i> that provokes liver and spleen inflammation and tissue destruction. In cutaneous leishmaniasis, the protein of <i>L. major</i>, named inhibitor of serine peptidases (ISP) 2, inactivates neutrophil elastase (NE) present at the macrophage surface, resulting in blockade of TLR4 activation, prevention of TNF-α and IFN-β production, and parasite survival. We report poor intracellular growth of <i>L. donovani</i> in macrophages from knockout mice for NE (<i>ela</i><sup>-/-</sup>), TLR4, or TLR2. NE and TLR4 colocalized with the parasite in the parasitophorous vacuole. Parasite load in the liver and spleen of <i>ela</i><sup>-/-</sup> mice were reduced and accompanied by increased NO and decreased TGF-β production. Exp"],"journal":["FASEB journal : official publication of the Federation of American Societies for Experimental Biology"],"pubmed_title":["Neutrophil elastase promotes <i>Leishmania donovani</i> infection <i>via</i> interferon-β."],"pmcid":["PMC6766642"],"funding_grant_id":["MR/N017269/1","MR/P027989/1"],"pubmed_authors":["Mukhtar MM","Godinho JP","Mottram JC","Lima APCA","Calegari-Silva T","Dias BT","Faria MS","Dias-Teixeira KL","Lopes U"],"additional_accession":[]},"is_claimable":false,"name":"Neutrophil elastase promotes <i>Leishmania donovani</i> infection <i>via</i> interferon-β.","description":"Visceral leishmaniasis is a deadly illness caused by <i>Leishmania donovani</i> that provokes liver and spleen inflammation and tissue destruction. In cutaneous leishmaniasis, the protein of <i>L. major</i>, named inhibitor of serine peptidases (ISP) 2, inactivates neutrophil elastase (NE) present at the macrophage surface, resulting in blockade of TLR4 activation, prevention of TNF-α and IFN-β production, and parasite survival. We report poor intracellular growth of <i>L. donovani</i> in macrophages from knockout mice for NE (<i>ela</i><sup>-/-</sup>), TLR4, or TLR2. NE and TLR4 colocalized with the parasite in the parasitophorous vacuole. Parasite load in the liver and spleen of <i>ela</i><sup>-/-</sup> mice were reduced and accompanied by increased NO and decreased TGF-β production. Exp","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Oct","modification":"2025-04-21T23:28:05.406Z","creation":"2019-10-11T07:12:51Z"},"accession":"S-EPMC6766642","cross_references":{"pubmed":["31284755"],"doi":["10.1096/fj.201900524R"]}}