biostudies-literatureFootitt SBiotechnology and Biological Sciences Research Council2325-2339https://www.ebi.ac.uk/biostudies/studies/S-EPMC6767449biostudies-literatureUnknown42(8)Aquaporins influence water flow in plants, yet little is known of their involvement in the water-driven process of seed germination. We therefore investigated their role in seeds in the laboratory and under field and global warming conditions. We mapped the expression of tonoplast intrinsic proteins (TIPs) during dormancy cycling and during germination under normal and water stress conditions. We found that the two key tonoplast aquaporins, TIP3;1 and TIP3;2, which have previously been implicated in water or solute transport, respectively, act antagonistically to modulate the response to abscisic acid, with TIP3;1 being a positive and TIP3;2 a negative regulator. A third isoform, TIP4;1, which is normally expressed upon completion of germination, was found to play an earlier role during waPlant, cell & environmentAquaporins influence seed dormancy and germination in response to stress.PMC6767449BB/J017582/11782597Footitt SFinch-Savage WEFrigerio LFeeney MClewes RfalseAquaporins influence seed dormancy and germination in response to stress.Aquaporins influence water flow in plants, yet little is known of their involvement in the water-driven process of seed germination. We therefore investigated their role in seeds in the laboratory and under field and global warming conditions. We mapped the expression of tonoplast intrinsic proteins (TIPs) during dormancy cycling and during germination under normal and water stress conditions. We found that the two key tonoplast aquaporins, TIP3;1 and TIP3;2, which have previously been implicated in water or solute transport, respectively, act antagonistically to modulate the response to abscisic acid, with TIP3;1 being a positive and TIP3;2 a negative regulator. A third isoform, TIP4;1, which is normally expressed upon completion of germination, was found to play an earlier role during wa2019-01-01T00:00:00Z2019 Aug2025-04-18T17:12:15.316Z2019-10-11T07:13:15ZS-EPMC67674493098689110.1111/pce.13561