biostudies-literatureHua DNational Institute of General Medical SciencesNIGMS NIH HHS11070-11077https://www.ebi.ac.uk/biostudies/studies/S-EPMC6768561biostudies-literatureUnknown91(17)"The totality is not, as it were, a mere heap, but the whole is something besides the parts."-Aristotle. We built a classifier that uses the totality of the glycomic profile, not restricted to a few glycoforms, to differentiate samples from two different sources. This approach, which relies on using thousands of features, is a radical departure from current strategies, where most of the glycomic profile is ignored in favor of selecting a few features, or even a single feature, meant to capture the differences in sample types. The classifier can be used to differentiate the source of the material; applicable sources may be different species of animals, different protein production methods, or, most importantly, different biological states (disease vs healthy). The classifier can be used on Analytical chemistryThe Aristotle Classifier: Using the Whole Glycomic Profile To Indicate a Disease State.PMC6768561R01GM103547R01 GM103547R35 GM130354R35GM130354Hua DDesaire HGo EPPatabandige MWfalseThe Aristotle Classifier: Using the Whole Glycomic Profile To Indicate a Disease State."The totality is not, as it were, a mere heap, but the whole is something besides the parts."-Aristotle. We built a classifier that uses the totality of the glycomic profile, not restricted to a few glycoforms, to differentiate samples from two different sources. This approach, which relies on using thousands of features, is a radical departure from current strategies, where most of the glycomic profile is ignored in favor of selecting a few features, or even a single feature, meant to capture the differences in sample types. The classifier can be used to differentiate the source of the material; applicable sources may be different species of animals, different protein production methods, or, most importantly, different biological states (disease vs healthy). The classifier can be used on 2019-01-01T00:00:00Z2019 Sep2025-04-22T10:34:11.25Z2025-04-22T10:34:11.25ZS-EPMC67685613140789310.1021/acs.analchem.9b01606