{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Clark BS"],"funding":["Chan-Zuckerberg Initiative DAF","NYSTEM","NEI NIH HHS","Johns Hopkins University","NINDS NIH HHS","NCI NIH HHS","NIH","Johns Hopkins University School of Medicine Synergy Award"],"pagination":["1111-1126.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6768831"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["102(6)"],"pubmed_abstract":["Precise temporal control of gene expression in neuronal progenitors is necessary for correct regulation of neurogenesis and cell fate specification. However, the cellular heterogeneity of the developing CNS has posed a major obstacle to identifying the gene regulatory networks that control these processes. To address this, we used single-cell RNA sequencing to profile ten developmental stages encompassing the full course of retinal neurogenesis. This allowed us to comprehensively characterize changes in gene expression that occur during initiation of neurogenesis, changes in developmental competence, and specification and differentiation of each major retinal cell type. We identify the NFI transcription factors (Nfia, Nfib, and Nfix) as selectively expressed in late retinal progenitor cell"],"journal":["Neuron"],"pubmed_title":["Single-Cell RNA-Seq Analysis of Retinal Development Identifies NFI Factors as Regulating Mitotic Exit and Late-Born Cell Specification."],"pmcid":["PMC6768831"],"funding_grant_id":["K08EY027093","R01 EY020560","U01 EY027267","U01EY027267","Discovery","C030133","P30 NS050274","C026429","K99 EY027844","R01EY020560","K99EY027844","R01 CA177669","U01CA212007","U01 CA212007","F32 EY024201","R01CA177669","C30290GG","K08 EY027093","2018-183444","Catalyst","F32EY024201","2018-183445"],"pubmed_authors":["Hoang TV","Santiago CP","Stein-O'Brien GL","Cannon GH","Rajaii F","James-Esposito RE","Clark BS","Blackshaw S","Davis-Marcisak E","Sherman T","Fertig EJ","Shiau F","Gronostajski RM","Goff LA"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell RNA-Seq Analysis of Retinal Development Identifies NFI Factors as Regulating Mitotic Exit and Late-Born Cell Specification.","description":"Precise temporal control of gene expression in neuronal progenitors is necessary for correct regulation of neurogenesis and cell fate specification. However, the cellular heterogeneity of the developing CNS has posed a major obstacle to identifying the gene regulatory networks that control these processes. To address this, we used single-cell RNA sequencing to profile ten developmental stages encompassing the full course of retinal neurogenesis. This allowed us to comprehensively characterize changes in gene expression that occur during initiation of neurogenesis, changes in developmental competence, and specification and differentiation of each major retinal cell type. We identify the NFI transcription factors (Nfia, Nfib, and Nfix) as selectively expressed in late retinal progenitor cell","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2026-05-04T16:38:08.028Z","creation":"2020-06-25T07:27:30Z"},"accession":"S-EPMC6768831","cross_references":{"pubmed":["31128945"],"doi":["10.1016/j.neuron.2019.04.010"]}}